Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 525,978 T→G S64A (TCT→GCT)  SAUSA300_RS02510 → methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793525,9780TG99.5% 500.8 / ‑5.6 192S64A (TCT→GCT) SAUSA300_RS02510methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (189/2);  minor base A (1/0);  total (190/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

Only 100 of 192 total aligned reads displayed.

GTTATTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAATCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAA  >  NC_007793/525882‑526074
                                                                                                 |                                                                                                
gTTATTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTg                                                                                               >  1:3495006/1‑100 (MQ=255)
   aTTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTa                                                                                            >  1:6143778/1‑100 (MQ=255)
      gCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAGATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGa                                                                                         >  1:6264810/1‑100 (MQ=255)
      gCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGa                                                                                         >  1:3647941/1‑100 (MQ=255)
      gCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGa                                                                                         >  1:2261110/1‑100 (MQ=255)
       gtaGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGAc                                                                                        >  1:3105123/3‑100 (MQ=255)
        caGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACa                                                                                       >  1:2365835/2‑100 (MQ=255)
         aGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAg                                                                                      >  1:5563391/1‑100 (MQ=255)
         aGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAg                                                                                      >  1:587392/1‑100 (MQ=255)
          gctataAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGa                                                                                     >  1:5298184/3‑100 (MQ=255)
               aaGAGAATGCAAGGATATGATGCTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAACTTg                                                                                >  1:6650087/1‑100 (MQ=255)
                gctgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGACAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGa                                                                               >  1:2662822/4‑100 (MQ=255)
                  tgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAGTTGaag                                                                             >  1:4235737/2‑99 (MQ=255)
                  tgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAat                                                                             >  1:1618150/2‑100 (MQ=255)
                  tgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAat                                                                             >  1:4286049/2‑100 (MQ=255)
                  tgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGACGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGatcggaaga                                                                             >  1:1007485/2‑92 (MQ=255)
                  agaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAACTGaaa                                                                             >  1:4199298/1‑99 (MQ=255)
                   taaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:3024880/2‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAAGCTGGTAAGACAGAAATTGAat                                                                             >  1:4166176/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGaagatcggaa                                                                            >  1:1826936/1‑92 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:5586653/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:2924634/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:1483052/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:4109165/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:4509917/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:6423438/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAACTGGTAAGACAGAAATTGAata                                                                            >  1:1743936/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGAAGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:4250624/1‑100 (MQ=255)
                   gaATGCAAGGATATGATGTTCACTATTTGACTGGAACGGATGGACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAata                                                                            >  1:3144344/1‑100 (MQ=255)
                   caaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGGC‑AAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAatat                                                                           >  1:2007109/2‑100 (MQ=255)
                    cttGCAAGGATATGATGTTCGCTATTTGACTGGCACGGCTGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑ACAAAGCTGGTAAGCCAGAAATTGAatat                                                                           >  1:3374831/3‑100 (MQ=255)
                    aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAACAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAatat                                                                           >  1:4663471/1‑100 (MQ=255)
                    aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAatat                                                                           >  1:5797133/1‑100 (MQ=255)
                    aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAatat                                                                           >  1:1396609/1‑100 (MQ=255)
                    aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAatat                                                                           >  1:1392658/1‑100 (MQ=255)
                    aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAatat                                                                           >  1:857164/1‑100 (MQ=255)
                     ctGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                          >  1:3197219/2‑100 (MQ=255)
                     aTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                          >  1:4434346/1‑100 (MQ=255)
                     aTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                          >  1:317079/1‑100 (MQ=255)
                     aTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                          >  1:5177676/1‑100 (MQ=255)
                      tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAATGCTGGTAAGACAGAAATTGAATAttt                                                                         >  1:2197375/1‑100 (MQ=255)
                      tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGCATAttt                                                                         >  1:3354924/1‑100 (MQ=255)
                      tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAttt                                                                         >  1:4321208/1‑100 (MQ=255)
                      tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAttt                                                                         >  1:1175174/1‑100 (MQ=255)
                      tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAttt                                                                         >  1:6113300/1‑100 (MQ=255)
                      tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAttc                                                                         >  1:3480989/1‑99 (MQ=255)
                      tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAttc                                                                         >  1:2526217/1‑99 (MQ=255)
                      agCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAatatat                                                                         >  1:320477/2‑98 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAttcg                                                                        >  1:5183509/1‑98 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATAttcg                                                                        >  1:628818/1‑98 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                        >  1:5513514/1‑100 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                        >  1:1848966/1‑100 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                        >  1:5159209/1‑100 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATCTg                                                                        >  1:6226828/1‑100 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAACTGAATATTTg                                                                        >  1:3567418/1‑100 (MQ=255)
                       gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAAAAATTGAATATTTg                                                                        >  1:4991273/1‑100 (MQ=255)
                          aGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgat                                                                     >  1:5071203/1‑100 (MQ=255)
                            agtatGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCGAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTCGgatga                                                                   >  1:6586900/3‑100 (MQ=255)
                            agtatGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAACATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAACTTGAATATTCGgacga                                                                   >  1:5662764/3‑100 (MQ=255)
                             ctatGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgag                                                                  >  1:1555902/2‑100 (MQ=255)
                             ctatGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgag                                                                  >  1:5015839/2‑100 (MQ=255)
                              tatGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgaga                                                                 >  1:3295596/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAGTATTCGgatgagat                                                                >  1:4850237/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGACTATTCGgatgagat                                                                >  1:4819611/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagat                                                                >  1:5503448/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagat                                                                >  1:1918105/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagat                                                                >  1:1709800/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagat                                                                >  1:2578609/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgaaga                                                                >  1:1589609/1‑97 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgataagat                                                                >  1:3917269/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTAgacgagat                                                                >  1:1682298/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgagat                                                                >  1:4464365/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgagat                                                                >  1:2868984/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTCGgacgagat                                                                >  1:787108/1‑100 (MQ=255)
                               atgatgTTCGCTATTTGACTGGAACGAATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgcgat                                                                >  1:2486102/1‑100 (MQ=255)
                                 gatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑‑AAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagatcgg                                                             >  1:2781180/1‑97 (MQ=255)
                                   tgctCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagatcgga                                                            >  1:2619811/4‑96 (MQ=255)
                                     ttCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagatcggaag                                                          >  1:3361394/1‑94 (MQ=255)
                                      ccGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGgacgagatagatcgg                                                         >  1:2986622/2‑93 (MQ=255)
                                       cGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTGGgatgagatcggaagag                                                        >  1:91719/1‑92 (MQ=255)
                                       cGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgagatcggaagag                                                        >  1:1109870/1‑92 (MQ=255)
                                            cttGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAAtt                                                   >  1:6037478/2‑100 (MQ=255)
                                              agccTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTaa                                                 >  1:5715180/4‑100 (MQ=255)
                                               gACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGagatcgg                                                >  1:5815159/1‑94 (MQ=255)
                                               gACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTaaa                                                >  1:5978676/1‑100 (MQ=255)
                                               gACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTaaa                                                >  1:361904/1‑100 (MQ=255)
                                                 cTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACa                                              >  1:1898841/1‑100 (MQ=255)
                                                  tGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACaa                                             >  1:1122765/1‑100 (MQ=255)
                                                   ggAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAAt                                            >  1:2742391/1‑100 (MQ=255)
                                                   ggAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAAt                                            >  1:3140984/1‑100 (MQ=255)
                                                            gAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTa                                   >  1:1023503/1‑100 (MQ=255)
                                                            gAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTa                                   >  1:4514917/1‑100 (MQ=255)
                                                            ctacacGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTa                                   >  1:3830643/3‑100 (MQ=255)
                                                            atacacGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTa                                   >  1:4374942/3‑100 (MQ=255)
                                                             tacacGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGcaag                                  >  1:2518241/2‑97 (MQ=255)
                                                              acacGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGTGATGATTGCTGGAATTAAACAATTGTGGGCTAAg                                 >  1:2355488/1‑100 (MQ=255)
                                                                 cGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCtt                              >  1:4995633/1‑100 (MQ=255)
                                                                                          c‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCa     >  1:2015398/1‑100 (MQ=255)
                                                                                          c‑AAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCa     >  1:3944380/1‑100 (MQ=255)
                                                                                              aaaGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGaa  >  1:3714460/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTTATTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTC‑AAAAATCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAA  >  NC_007793/525882‑526074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑