Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	519,306	T→C	F169S (TTT→TCT)	SAUSA300_RS02455 →	lysine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	519,306	0	T	C	100.0%	40.6 / NA	17	F169S (TTT→TCT)	SAUSA300_RS02455	lysine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/0);  total (17/0)

AATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATTTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTGGATTGCAAGGATTTCCAGATTC  >  NC_007793/519227‑519384                                                                                |                                                                               aatGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTc                                                            >  1:2433508/1‑100 (MQ=255)    accGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTg                                                         >  1:1668205/3‑100 (MQ=255)       taccacaAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTAc                                                      >  1:1172399/4‑100 (MQ=255)        ctCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACt                                                     >  1:1114980/2‑100 (MQ=255)              ctcaggCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGa                                               >  1:2062402/6‑100 (MQ=255)              aaCTTCCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGa                                               >  1:3569414/1‑100 (MQ=255)               aCTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGttagatcggaa                                              >  1:946472/1‑90 (MQ=255)               aCTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGAc                                              >  1:483168/1‑100 (MQ=255)                  tGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCAttagatcg                                           >  1:1713284/1‑94 (MQ=255)                  tGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGaa                                           >  1:292419/1‑100 (MQ=255)                  tGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGaa                                           >  1:3165669/1‑100 (MQ=255)                  tGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGaa                                           >  1:5215020/1‑100 (MQ=255)                  tGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGaa                                           >  1:5289911/1‑100 (MQ=255)                  tGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACCCATTGagatc                                           >  1:4030502/1‑96 (MQ=255)                                  ccTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACt                           >  1:4627276/1‑100 (MQ=255)                                     aaCTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTg                        >  1:5854190/1‑100 (MQ=255)                                                           ccctAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTGGATTGCAAGGATTTCCAGATTc  >  1:6038026/4‑100 (MQ=255)                                                                                |                                                                               AATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATTTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTGGATTGCAAGGATTTCCAGATTC  >  NC_007793/519227‑519384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑