Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,872,409	T→A	intergenic (‑91/+36)	rnpA ← / ← SAUSA300_RS14700	ribonuclease P protein component/50S ribosomal protein L34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,872,409	0	T	A	100.0%	103.5 / NA	36	intergenic (‑91/+36)	rnpA/SAUSA300_RS14700	ribonuclease P protein component/50S ribosomal protein L34
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (1/35);  total (1/35)

CATCACTCGCTTATTTATCGTTTTTATTTGAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTTAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGCCATTT  >  NC_007793/2872322‑2872505                                                                                        |                                                                                                 cATCACTCGCTTATTTATCGTTTTTATTTGAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGaaaaaaa                                                                                      <  1:1254900/100‑1 (MQ=255)    cACTCGCTTATTTATCGTTTTTATTTGAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGa                                                                                   >  1:229150/1‑100 (MQ=255)                 atCGTTTTTATTTGAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTc                                                                      <  1:1194318/100‑1 (MQ=255)                  tCGTTTTTATTTGAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTct                                                                     <  1:492515/100‑2 (MQ=255)                              gAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAGAGATCACTGATGAGTCAGTGATCTTATGc                                                         <  1:908656/100‑1 (MQ=255)                              gAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGc                                                         <  1:4595397/100‑1 (MQ=255)                              gAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGc                                                         <  1:3691538/100‑1 (MQ=255)                              gAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGc                                                         <  1:3682667/100‑1 (MQ=255)                              gAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTGAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGc                                                         <  1:5453961/100‑1 (MQ=255)                                 gCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAgt                                                      <  1:202745/100‑2 (MQ=255)                                  cTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGaa                                                     <  1:4174198/100‑2 (MQ=255)                                              ttCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACgg                                         <  1:731627/100‑1 (MQ=255)                                                 tATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCt                                      <  1:6636207/100‑1 (MQ=255)                                                 tATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCt                                      <  1:6148653/100‑1 (MQ=255)                                                 tATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCt                                      <  1:3614939/100‑1 (MQ=255)                                                  aTTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGccaa                                     <  1:1079338/100‑3 (MQ=255)                                                  aTTGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCtg                                     <  1:24655/100‑2 (MQ=255)                                                   ttGAGCTTATACAATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCttt                                    <  1:1304022/100‑1 (MQ=255)                                                   ttGAGCTTATAACATAAATTTCTATTTATTGTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCttt                                    <  1:1897522/100‑1 (MQ=255)                                                   ttGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATTACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCttt                                    <  1:5338973/100‑1 (MQ=255)                                                   ttGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCttt                                    <  1:4521101/100‑1 (MQ=255)                                                   ttGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCttt                                    <  1:6229622/100‑1 (MQ=255)                                                   ttGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCtgg                                    <  1:3008024/100‑3 (MQ=255)                                                    tGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCtgag                                   <  1:1208446/100‑4 (MQ=255)                                                    tGAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCGTATGCAGATAAAACTTTACGGCCttgg                                   <  1:6545893/100‑3 (MQ=255)                                                     gAGCTTATAACATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTac                                  <  1:204827/100‑1 (MQ=255)                                                              aCATAAATTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGgc                         <  1:2986211/100‑1 (MQ=255)                                                                    aTTTCTATTTATTCTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTa                   <  1:5553341/100‑1 (MQ=255)                                                                          atttattCTTAATTAAACCGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACtt             <  1:488089/100‑1 (MQ=255)                                                                                 cTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGcc      <  1:1551268/100‑1 (MQ=255)                                                                                 cTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGcc      <  1:3325018/100‑1 (MQ=255)                                                                                 cTTAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGCGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGcg      <  1:2672634/100‑2 (MQ=255)                                                                                   tAATTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGctgt    <  1:3396709/100‑4 (MQ=255)                                                                                    aaTTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGCCAtt   <  1:3163876/100‑1 (MQ=255)                                                                                    aaTTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGCCAtc   <  1:5620513/100‑2 (MQ=255)                                                                                     aTTAAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGCCAtta  <  1:3510780/100‑2 (MQ=255)                                                                                        |                                                                                                 CATCACTCGCTTATTTATCGTTTTTATTTGAAGCTATATTTAAACTTCTATTGAGCTTATAACATAAATTTCTATTTATTCTTAATTTAAACGAAAAAAAAGATCACTGATGAGTCAGTGATCTTATGCAGATAAAACTTTACGGCCTTTACGACGACGGCGCGCTAAAACTTTACGGCCATTT  >  NC_007793/2872322‑2872505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑