Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	508,670	A→G	intergenic (+71/‑67)	SAUSA300_RS02410 → / → SAUSA300_RS02415	hypothetical protein/acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	508,670	0	A	G	100.0%	51.1 / NA	20	intergenic (+71/‑67)	SAUSA300_RS02410/SAUSA300_RS02415	hypothetical protein/acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/0);  total (20/0)

CGATGTGCAGTTTGAATGATTTTTGTGTCAATGAAAAGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGGAGTATCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTACTTTA  >  NC_007793/508581‑508760                                                                                          |                                                                                           cGATGTGCAGTTTGAATGATTTTTGTGTCAATGAAAAGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGGAGTGTCTTACAATa                                                                                  >  1:1438681/1‑100 (MQ=255) cGATGTGCAGTTTGAATGATTTTTGTGTCAATGAAAAGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGAAGTGTCTTACAATa                                                                                  >  1:4881549/1‑100 (MQ=255)    tgtgCAGTTTGAATGATTTTTGTGTCAATGAAAAGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGGAGTGTCTTACAATAGaa                                                                               >  1:2608124/1‑100 (MQ=255)       gCAGTTTGAATGATTTTTGTGTCAATGAAAAGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGGAGTGTCTTACAATAGAatta                                                                            >  1:1259587/1‑100 (MQ=255)                             cAATGAAAAGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGatta                                                      >  1:3535814/1‑100 (MQ=255)                                  aaaaGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGAc                                                 >  1:5880015/1‑100 (MQ=255)                                                                 caaTGATGGTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAat                  >  1:6269219/2‑100 (MQ=255)                                                                      ctgGTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATAtt             >  1:1321970/2‑100 (MQ=255)                                                                       tgGTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATAttt            >  1:1696575/1‑100 (MQ=255)                                                                        ggTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTa           >  1:1229435/1‑100 (MQ=255)                                                                        ggTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTa           >  1:5694072/1‑100 (MQ=255)                                                                        ggTGAAATGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTa           >  1:1016122/1‑100 (MQ=255)                                                                            acctGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTa       >  1:4158709/4‑100 (MQ=255)                                                                             aaTGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTAc      >  1:504189/1‑100 (MQ=255)                                                                              ttGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTACt     >  1:3515019/2‑100 (MQ=255)                                                                              gtGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTACt     >  1:1353017/2‑100 (MQ=255)                                                                               tatgggggAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTACtt    >  1:102266/4‑100 (MQ=255)                                                                               tGAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTACtt    >  1:1351789/1‑100 (MQ=255)                                                                                gAGGGGGAGTGTCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTACttt   >  1:2573114/1‑100 (MQ=255)                                                                                 aGGGGGAGTGTCTTACAATAGAATTATTAATGAGAGACGTTATGATTATTGACAATCAAATGACTACGGAGGACAGATGCAAATATATTTAAGTAATTTa  >  1:278885/1‑100 (MQ=255)                                                                                          |                                                                                           CGATGTGCAGTTTGAATGATTTTTGTGTCAATGAAAAGTAAGAAGTTATAATTTGATGATAAAGAAATGATGGTGAAATGAGGGGGAGTATCTTACAATAGAATTATTAATGAGATACGTTATGATTATTGACAATCAAATGCCTACGGAGGACATATGCAAATATATTTAAGTACTTTA  >  NC_007793/508581‑508760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑