Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,839,280	C→A	G140C (GGT→TGT)	hisG ←	ATP phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,839,280	0	C	A	100.0%	131.8 / NA	47	G140C (GGT→TGT)	hisG	ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (1/46);  total (1/46)

ATCACTAATATGTTGCTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACCGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCGAT  >  NC_007793/2839185‑2839376                                                                                                |                                                                                                 atCACTAATATGTTGCTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGcc                                                                                              <  1:5502209/100‑1 (MQ=255)  atcttAATATGTTGCTTTTCAACCAGCCCGTTCGCATTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGCCa                                                                                             <  1:4386062/96‑1 (MQ=255)   cACTAATATGTTGCTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGCCag                                                                                            <  1:2201345/100‑2 (MQ=255)    aCTAATATGTTGCTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGCCaag                                                                                           <  1:3474770/100‑2 (MQ=255)    aCTAATATGTTGCTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGCCAAt                                                                                           <  1:604472/100‑1 (MQ=255)               gCTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGCCAATTCAACAGAGcc                                                                                <  1:4472385/100‑1 (MQ=255)                cTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCa                                                                               <  1:2704930/100‑1 (MQ=255)                                              agtaCCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCt                                                 <  1:2500146/100‑1 (MQ=255)                                                                  cGACAATTCCGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAAACAATTCGACATCAATACCTTTTGATTTAAAATATGTTTc                             <  1:5335986/100‑1 (MQ=255)                                                                     tcttccgatCTTCCATGTCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:327460/92‑1 (MQ=255)                                                                     tcttccgatCTATCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:1265940/92‑1 (MQ=255)                                                                     tcttccgatCTACCATGTCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:3785656/92‑1 (MQ=255)                                                                     tcttccgatCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:1858517/92‑1 (MQ=255)                                                                     tcttccgatCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:2247360/92‑1 (MQ=255)                                                                     tcttccgatCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:2722160/92‑1 (MQ=255)                                                                     tcttccgatCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:3152341/92‑1 (MQ=255)                                                                     cAATTCCGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagc                          <  1:247963/100‑1 (MQ=255)                                                                      cttccgatCTACCA‑ATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagcag                        <  1:6193921/93‑1 (MQ=255)                                                                       ttccgatCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTcagcag                        <  1:2498145/94‑1 (MQ=255)                                                                        ttCCGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGt                       <  1:4359114/100‑1 (MQ=255)                                                                        ttCCGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGt                       <  1:6435406/100‑1 (MQ=255)                                                                        tccgatCTACCTTATCTACAACACAGGCCAATTCAACAGAGCCATACAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGt                       <  1:5669489/95‑1 (MQ=255)                                                                        tccgatCTACC‑TATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTGGATTTAAAATATGTTTCAGCAGTa                      <  1:6490525/95‑1 (MQ=255)                                                                         gatctCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTa                      <  1:1180830/96‑1 (MQ=255)                                                                         cttgTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATTCCTTTTGATTTAAAATATGTTTCAGCAGTa                      <  1:1003567/97‑1 (MQ=255)                                                                         ccgatCTACCATTTCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTa                      <  1:5333069/96‑1 (MQ=255)                                                                         ccgatCTACCAGATTTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTa                      <  1:4969971/96‑1 (MQ=255)                                                                         ccgatCTACCACGTCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTa                      <  1:3414829/96‑1 (MQ=255)                                                                          cgatCTATCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTAt                     <  1:3118628/97‑1 (MQ=255)                                                                          cgatCTACCGCATCTACAACACAGGCCAATTCAGCAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTag                     <  1:2634297/97‑2 (MQ=255)                                                                          cgatCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTaa                     <  1:5626710/97‑2 (MQ=255)                                                                          cgatCTACCAGAACTACAACACAGGCCAATTCAACAGCGCCATTCAATTTAATCAATTCGACATCAATTCCTGTTGATTTAAAATATGTTTCAGCAGTag                     <  1:6088331/97‑2 (MQ=255)                                                                          cgatCTACC‑TATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATg                    <  1:5894073/97‑1 (MQ=255)                                                                          ccGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTac                     <  1:5012015/100‑2 (MQ=255)                                                                          ccGTCTACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTAt                     <  1:5675058/100‑1 (MQ=255)                                                                           gatctcCCATGTCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATg                    <  1:3686636/98‑1 (MQ=255)                                                                           gatCTACCTTATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATGCCTTTTGATTTAAAATATGTTTcagcagggca                    <  1:3059447/98‑5 (MQ=255)                                                                           gatCTACCGCATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATg                    <  1:5437512/98‑1 (MQ=255)                                                                           cGTCTACCATATCTACAACACAGGCCAATTCAACAGAGTCATTCAATTTAATCAATACGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATg                    <  1:5234639/100‑1 (MQ=255)                                                                           atcttACCATATCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATg                    <  1:4273938/96‑1 (MQ=255)                                                                               accactatCTACAACACAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACa                >  1:5052091/6‑100 (MQ=255)                                                                                        tACAACACAGGCCAATTCAACAGAGAAATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAAATCGtt       <  1:4105002/100‑1 (MQ=255)                                                                                           aacaCAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATGCCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCg    <  1:4003599/100‑1 (MQ=255)                                                                                           aacaCAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCg    <  1:3979517/100‑1 (MQ=255)                                                                                           aacaCAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCg    <  1:4456023/100‑1 (MQ=255)                                                                                           aacaCAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCg    <  1:3872081/100‑1 (MQ=255)                                                                                            acacAGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCgg   <  1:269992/100‑2 (MQ=255)                                                                                             cacaGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCGAt  <  1:2511288/100‑1 (MQ=255)                                                                                                |                                                                                                 ATCACTAATATGTTGCTTTTCAACCAGTCCGTTCGCTTTTAGCGTAGTACCTGTTTGAACGATGTCGACAATTCCGTCTACCATATCTACAACACCGGCCAATTCAACAGAGCCATTCAATTTAATCAATTCGACATCAATACCTTTTGATTTAAAATATGTTTCAGCAGTATGAACATAACTCGTTGCGAT  >  NC_007793/2839185‑2839376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑