Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,798,664 T→A E602V (GAA→GTA)  SAUSA300_RS14355 ← surface anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,798,6640TA95.5% 55.3 / NA 22E602V (GAA→GTA) SAUSA300_RS14355surface anchored protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (0/21);  minor base C (0/1);  total (0/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTTTTCAACTTATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTTCCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCACTTAACAATCCATTACCTTTACCAAGTGTTAACGTCTTTTCTGGCTTT  >  NC_007793/2798569‑2798759
                                                                                               |                                                                                               
tATTTTTCAACTTATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTAccca                                                                                             <  1:4732259/100‑2 (MQ=255)
   ttttCAACTTATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGc                                                                                          <  1:91182/100‑1 (MQ=255)
      tCAACTTATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCttt                                                                                       <  1:5095414/100‑1 (MQ=255)
         aCTTATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAgg                                                                                    <  1:124053/100‑1 (MQ=255)
           ttATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCa                                                                                  <  1:1950274/100‑1 (MQ=255)
           ttATTGCGTCGTGAGAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCa                                                                                  <  1:4907627/100‑1 (MQ=255)
            tactGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCaa                                                                                 <  1:5871638/97‑1 (MQ=255)
            tATTGCGTCGTGATATCCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCATCCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCaa                                                                                 <  1:6163447/100‑1 (MQ=255)
            tATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCat                                                                                 <  1:501707/100‑2 (MQ=255)
            tATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCag                                                                                 <  1:760248/100‑2 (MQ=255)
            tATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCaa                                                                                 <  1:2864252/100‑1 (MQ=255)
            tATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCaa                                                                                 <  1:2566932/100‑1 (MQ=255)
            tATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCaa                                                                                 <  1:3903920/100‑1 (MQ=255)
            tATTGCGTCGTGATAACCAAATCATTAGTACACCCAGTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCaa                                                                                 <  1:2947421/100‑1 (MQ=255)
            tATTGCGTCGTGATAACCAAATCATTAGTACACACATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCaa                                                                                 <  1:5466383/100‑1 (MQ=255)
            gaTTGCGTCGTGATAACCAAATCATGAGTACGCCCATTGCGCCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTCCCCCCGCTTTAGGCaa                                                                                 <  1:3794868/99‑1 (MQ=255)
                                                             tACAGATATCGGCAACCAATGTTCTTTTATCGTTACCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCACTTAACAATCCATTACCt                                <  1:3809832/100‑1 (MQ=255)
                                                                                        tATCGTTACCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCACTTAACAATCCATTACCTTTACCAAGTGTTAACGTCTTTTCTggg     <  1:5275449/100‑2 (MQ=255)
                                                                                        tATCGTTACCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCACTTAACAATCCATTACCTTTACCAAGTGTTAACGTCTTTTCTGGc     <  1:3808149/100‑1 (MQ=255)
                                                                                        gaTCTTTACCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCACTTAACAATCCATTACCTTTACCAAGTGTTAACGTCTTTTCTGGc     <  1:5313291/99‑1 (MQ=255)
                                                                                        atctgTTACCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCACTTAACAATCCATTACCTTTACCAAGTGTTAACGTCTTTTCTGGc     <  1:5194478/96‑1 (MQ=255)
                                                                                           cGTTACCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCGCTTAACAATCCATTACCTTTACCAAGTGTTAACGTCTTTTCTGGCttt  <  1:3148913/100‑1 (MQ=255)
                                                                                               |                                                                                               
TATTTTTCAACTTATTGCGTCGTGATAACCAAATCATTAGTACACCCATTGCACCAACAATTACAGATATCGGCAACCAATGTTCTTTTATCGTTTCCCCCGCTTTAGGCAAAGATACATTACCATCAGCATTTAATAATCCACTTAACAATCCATTACCTTTACCAAGTGTTAACGTCTTTTCTGGCTTT  >  NC_007793/2798569‑2798759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑