Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,775,724	A→G	I439T (ATA→ACA)	SAUSA300_RS14270 ←	clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,775,724	0	A	G	100.0%	209.2 / NA	79	I439T (ATA→ACA)	SAUSA300_RS14270	clumping factor B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/76);  total (3/76)

GGATGCTCATAATAGATTCTATTTTTAAATTGGTCTGTTACTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATATATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTAATATACACCC  >  NC_007793/2775628‑2775820                                                                                                 |                                                                                                 ggATGCTCATAATAGATTCTATTTTTAAATTGGTCTGTTACTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGatgtat                                                                                               <  1:849419/100‑1 (MQ=255) ggATGCTCATAATAGATTCTATTTTTAAATTGGTCTGTTACTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGatgtat                                                                                               <  1:3901949/100‑1 (MQ=255)                                   tccgatcttcttTGAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCGCTTCAAAAATTCTCAGTTTTGTATCTGt                                                             <  1:2548116/94‑1 (MQ=255)                                   cagTTGCTTCTTCAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCAATCTCTTCAAAAATTCTCAGTTTTGTAGCTGt                                                             <  1:4126248/98‑1 (MQ=255)                                   cTGTTACTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGt                                                             <  1:98621/100‑1 (MQ=255)                                    tGTTGCTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGtg                                                            <  1:6085198/100‑2 (MQ=255)                                     gTTACTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAg                                                           <  1:2097109/100‑1 (MQ=255)                                      ttACTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGc                                                          <  1:2827718/100‑1 (MQ=255)                                       tgcttcttTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCa                                                         <  1:2978520/98‑1 (MQ=255)                                          ttcttTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCactt                                                      <  1:3966313/100‑1 (MQ=255)                                           tcttTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGccgcca                                                     <  1:3040140/100‑6 (MQ=255)                                           tcttTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCactta                                                     <  1:246367/100‑1 (MQ=255)                                              ttcgggTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCacttactt                                                  >  1:1042939/5‑100 (MQ=255)                                              ttAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCacttactt                                                  >  1:6186664/1‑100 (MQ=255)                                               tAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACttt                                                 <  1:6062220/100‑1 (MQ=255)                                                 aGGTTAGAGTCATTCGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTAc                                               <  1:4984049/100‑1 (MQ=255)                                                  ggTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTAcc                                              <  1:6035091/100‑1 (MQ=255)                                                   gTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTAcct                                             <  1:8094/100‑2 (MQ=255)                                                     tAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCt                                           <  1:865221/100‑1 (MQ=255)                                                     tAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCt                                           <  1:436046/100‑1 (MQ=255)                                                     tAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCt                                           <  1:2128932/100‑1 (MQ=255)                                                     tAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCt                                           <  1:2138440/100‑1 (MQ=255)                                                        tgctcttccgATCTGC‑TAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACtt                                       <  1:2724054/91‑1 (MQ=255)                                                             ttccgATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTttctt                                   <  1:6431111/96‑1 (MQ=255)                                                                 gATCTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGAt                               <  1:4708846/100‑1 (MQ=255)                                                                    cTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCggggag                            <  1:561548/100‑6 (MQ=255)                                                                    cTGCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGGtttt                            <  1:5967003/100‑1 (MQ=255)                                                                      gtttagtagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACCTTCTTCGATTTTAt                          >  1:6434272/4‑100 (MQ=255)                                                                      gCATAGTAGCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCgcgggtgc                          <  1:2578393/100‑8 (MQ=255)                                                                           gtagCTATCTGATAATTTAGATGTATCATTCACTTC‑AAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:3399966/100‑1 (MQ=255)                                                                            tggCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTggc                    <  1:4783988/98‑2 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTTGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:1204887/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:6063623/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:5371307/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:1977801/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:2084138/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:5146166/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:6137031/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:5788200/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:2099355/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:6158370/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:6039013/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:481965/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:5997571/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:4382924/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:1525505/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:2786723/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:7422/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:2635889/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:325889/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:3303698/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:3330545/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:3788325/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:6266438/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:1538081/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:4328357/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:4661834/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGACTTTATCTTGGt                    <  1:3932429/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTACTTCGATTTTATCTTGGt                    <  1:3870826/100‑1 (MQ=255)                                                                            tagCTATCTGATAATTTAGATGTATCATTCACTCCAAAAATTCTCAGTTTTGTATCTGTAGCACTTGCTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:6051560/100‑1 (MQ=255)                                                                            tagCGATCTGATAATTTAGATGTATCATTCACTTCAAAAATTTTCAGTTTTGTATCTGTAGCACTTTCTTTACCGCTACTTTCTTCGATTTTATCTTGGt                    <  1:836232/100‑1 (MQ=255)                                                                             agCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTa                   <  1:5966822/100‑1 (MQ=255)                                                                             agCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTa                   <  1:4053530/100‑1 (MQ=255)                                                                              gCTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAg                  <  1:4131448/100‑1 (MQ=255)                                                                               cTATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAgt                 <  1:3877018/100‑2 (MQ=255)                                                                               cTATCTGATAATTTAGATGTATCATTCACTTC‑AAAATTCTCAGTTTTGTATCTGTAGCAATTTCTGTACCGCTTCTTTCTTCGATTTTATCTTGGTAGcc                <  1:4829206/100‑1 (MQ=255)                                                                                tATCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGca                <  1:5476446/100‑2 (MQ=255)                                                                                 aTCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCt               <  1:1513182/100‑1 (MQ=255)                                                                                 aTCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCt               <  1:1131572/100‑1 (MQ=255)                                                                                  tCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCtt              <  1:1559509/100‑1 (MQ=255)                                                                                  tCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCtt              <  1:2648664/100‑1 (MQ=255)                                                                                  tCTGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCtg              <  1:2524667/100‑2 (MQ=255)                                                                                    tGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCttag            <  1:5985853/100‑3 (MQ=255)                                                                                    tGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCtgga            <  1:6608332/100‑4 (MQ=255)                                                                                    tGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTa            <  1:5467252/100‑1 (MQ=255)                                                                                    tGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTa            <  1:5049044/100‑1 (MQ=255)                                                                                    tGATAATTTAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTa            <  1:2522694/100‑1 (MQ=255)                                                                                          tttAGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTAATATac      <  1:5234880/100‑1 (MQ=255)                                                                                           ctcgATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACCTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTAATATaca     <  1:6226508/97‑1 (MQ=255)                                                                                             aGATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTAATATacagc   <  1:799609/100‑3 (MQ=255)                                                                                              gATGTATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTCTCTTCGATTTTATCTTGGTAGCCTTTAATATACAccc  <  1:2774884/100‑1 (MQ=255)                                                                                                 |                                                                                                 GGATGCTCATAATAGATTCTATTTTTAAATTGGTCTGTTACTTCTTTAAGGTTAGAGTCATTTGGATCTGCATAGTAGCTATCTGATAATTTAGATATATCATTCACTTCAAAAATTCTCAGTTTTGTATCTGTAGCACTTACTTTACCGCTACTTTCTTCGATTTTATCTTGGTAGCCTTTAATATACACCC  >  NC_007793/2775628‑2775820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑