Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,759,623	T→C	P410P (CCA→CCG)	SAUSA300_RS14180 ←	citrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,759,623	0	T	C	100.0%	67.9 / NA	29	P410P (CCA→CCG)	SAUSA300_RS14180	citrate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/26);  total (3/26)

AGCGAATCCCCAAATCCAAAAGAATGCATACTTAATATACGTGCCCATGTTTGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAATGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCTGTTTG  >  NC_007793/2759530‑2759709                                                                                              |                                                                                       aGCGAATCCCCAAATCCAAAAGAATGCATCCTTAATATACGTGCCCATGTTTGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCtt                                                                                  <  1:4350906/100‑2 (MQ=255)            aaaTCCAAAAGAATGCATACTTAATATACGTGCCCATGTTTGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTAcg                                                                       <  1:4039164/100‑2 (MQ=255)             taTCCAAAAGAATGCATACTTAATATACGTGCCCATGTTTGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCt                                                                      <  1:3148075/99‑1 (MQ=255)              aTCCAAAAGAATGCATACTTAATATACGTGCCCATGTTTGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCta                                                                     <  1:1047559/100‑1 (MQ=255)                          gCATACTTAATATACGTGCCCATGTTTGCCTCTGCTAAACCAATTGCCACCCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTa                                                         >  1:1504124/1‑100 (MQ=255)                                              ttccgatctgtctGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGtt                                     <  1:4624952/90‑1 (MQ=255)                                               cgatctGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTg                                    <  1:2755581/95‑1 (MQ=255)                                                 tcttGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGac                                  <  1:6522587/98‑2 (MQ=255)                                                 tcttGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGAt                                  <  1:3279509/98‑1 (MQ=255)                                                 gTTTGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGAt                                  <  1:4981016/100‑1 (MQ=255)                                                   gatctactGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATAc                                <  1:6108946/94‑1 (MQ=255)                                                     ctctctGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACat                              <  1:1284010/98‑2 (MQ=255)                                                           gCTAAACCAATTGCCAACCATATGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGACACAGatcgga                        >  1:2304669/1‑95 (MQ=255)                                                            cTAAACCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTac                       <  1:1965768/100‑1 (MQ=255)                                                              aaaCCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTacac                     >  1:682884/1‑100 (MQ=255)                                                               aaCCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACAcc                    <  1:570476/100‑1 (MQ=255)                                                               aaCCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACAcc                    <  1:1232243/100‑1 (MQ=255)                                                                aCCAATTGCCAACCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCa                   <  1:4208301/100‑1 (MQ=255)                                                                          aaCCATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCcta         <  1:5936217/100‑2 (MQ=255)                                                                            ccATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATCATATTCCCTATGACCATTGAAAAAGCCGTTGATACAGACGGTACACCAAATTGCCctgag       <  1:316821/100‑3 (MQ=255)                                                                            ccATAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCctgct       <  1:1775055/100‑1 (MQ=255)                                                                              agaaGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTAGATACAGACGGTACTCCAAATTGCCCTGCtgt     <  1:6008533/98‑1 (MQ=255)                                                                              aTAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCtgt     <  1:4330779/100‑1 (MQ=255)                                                                              aTAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCtgt     <  1:2312364/100‑1 (MQ=255)                                                                              aTAAGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCtgt     <  1:1535394/100‑1 (MQ=255)                                                                               tAAGGCTGGTGAAGACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCtgtt    <  1:5914727/100‑1 (MQ=255)                                                                                aaGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCtgtta   <  1:1055364/100‑2 (MQ=255)                                                                                aaGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCggtac   <  1:6407797/100‑3 (MQ=255)                                                                                 aGGCTGGTGAAAACGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCtgttct  <  1:2623168/100‑3 (MQ=255)                                                                                              |                                                                                       AGCGAATCCCCAAATCCAAAAGAATGCATACTTAATATACGTGCCCATGTTTGCCTCTGCTAAACCAATTGCCAACCATAAGGCTGGTGAAAATGGGCTGACAAATGTACCTATAATATTCCCTATGACCATTGAATAAGCTGTTGATACAGACGGTACACCAAATTGCCCTGCTGTTTG  >  NC_007793/2759530‑2759709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑