Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	503,675	A→T	noncoding (25/93 nt)	SAUSA300_RS02390 →	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	503,675	0	A	T	82.1%	49.4 / 4.1	31	noncoding (25/93 nt)	SAUSA300_RS02390	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base T (23/0);  total (31/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTGTGTTTAGGAGGAATACCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATA  >  NC_007793/503640‑503771                                    |                                                                                                 cttggtgtTTAGGAGGAATACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTtcctcc                                  >  1:6548318/5‑100 (MQ=255)  taggtgtTTAGGAGGAATACCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCg                                 >  1:1679574/4‑100 (MQ=25)  accgtgtTTAGGAGGAATACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAACCCCCCTTCCTCCg                                 >  1:5489035/4‑100 (MQ=11)   gtgtgTTTAGGAGGAATACCCAAGTCCGGCTGACGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGc                                >  1:2176906/2‑100 (MQ=255)     gtgtTTAGGAGGAATACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCa                              >  1:3889540/1‑100 (MQ=255)           gggaggaATACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAata                        >  1:1532848/2‑100 (MQ=255)                 caTACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCCTCCTCCGCCCTCAATAtttgaa                  >  1:4601946/2‑97 (MQ=14)                  aTACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATTCCTCTTCCTCCGCCACCaacgattactg                 >  1:5014696/1‑91 (MQ=11)                  aTACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTatat                 >  1:4241005/1‑100 (MQ=255)                  aTACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTatat                 >  1:3581952/1‑100 (MQ=255)                   tACCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTaaata                >  1:3014406/1‑99 (MQ=255)                    ccccAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATTCCTCTTCCTCCGCCATTAATACTTttatta               >  1:2782644/2‑100 (MQ=14)                    aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGATAGGGGCTTAACGGCTCGCGGGGGTTCGAAACCCTCTTCCTCCGCCATCAATATTTATACTa               >  1:3408870/1‑100 (MQ=17)                    aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATAtttgggatg               >  1:4250284/1‑94 (MQ=255)                    aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATAttaggaaac               >  1:160221/1‑93 (MQ=255)                    aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTtaataga               >  1:3410088/1‑95 (MQ=255)                    aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTagatct               >  1:2450226/1‑98 (MQ=255)                    aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAAACCCTCTTCCTCCGCCATCAATATTTATATTa               >  1:120431/1‑100 (MQ=255)                    aCCCAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCCAGGGGGTTTGAATCCCCATTCCTCCGCCATCAATATTTATATTa               >  1:1979620/1‑100 (MQ=17)                    aCCCAAGTCCGGCTGAGGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTtatagtg               >  1:2569074/1‑97 (MQ=255)                    aCCCAAGTCCGGCTGAGGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATACCTCTTCCTCCGCCATCAATATTTatataa               >  1:3076171/1‑98 (MQ=255)                    aCCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTtaaaggg               >  1:689553/1‑95 (MQ=255)                      ccAAGTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTtataagcag             >  1:2122981/1‑95 (MQ=255)                         ggTCCGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTc          >  1:789332/2‑100 (MQ=255)                         cctCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGGTCCCGGGGGTTCGAAACCCTCTTCCTCCGCCCTCAATATTTATATTAAATTc          >  1:1715211/3‑100 (MQ=17)                           cccGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCta        >  1:187642/2‑100 (MQ=255)                           cccGGCTGAAGGGATCGGGCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCta        >  1:4116165/2‑100 (MQ=255)                             tggCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGTGGGGGTTCGAATACCTCTTCCTCCGCCATCAATATTTATATTAAATTCtata      >  1:2238565/2‑100 (MQ=255)                             cGGCTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtata      >  1:6408688/1‑100 (MQ=255)                                cTGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCtatatat   >  1:4923522/1‑100 (MQ=255)                                 tGATGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGGTCGAATTCCTCTTCCTCCGCCATCAATATTTTTATTAAATTCtatattta  >  1:4452297/1‑100 (MQ=18)                                    |                                                                                                 TATTGTGTTTAGGAGGAATACCCAAGTCCGGCTGAAGGGATCGGTCTTGAAAACCGACAGGGGCTTAACGGCTCGCGGGGGTTCGAATCCCTCTTCCTCCGCCATCAATATTTATATTAAATTCTATATATA  >  NC_007793/503640‑503771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑