Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,733,322	G→T	E488D (GAG→GAT)	SAUSA300_RS14045 →	acyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,733,322	0	G	T	100.0%	27.4 / NA	12	E488D (GAG→GAT)	SAUSA300_RS14045	acyl esterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/0);  total (12/0)

ATCACATCGTGAATTAGATCAAGAAAAATCCTCAATCGCGCAACCTTGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGAGATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGAAACATTGGAAGTTGTTGTAAAGGGT  >  NC_007793/2733235‑2733403                                                                                        |                                                                                  ctCACATCGTGAATTAGATCAAGAAAAATCCTCAATCGCGCAACCTTGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGtt                                                                       >  1:386471/2‑100 (MQ=255)                      aGAAAAATCCTCAATCGCGCAACCTTGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCa                                                  >  1:4753849/1‑100 (MQ=255)                                 cAATCGCGCAACCTTGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTAtt                                       >  1:2590063/1‑100 (MQ=255)                                      ccgcAACCTTGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAAc                                  >  1:6247530/2‑100 (MQ=255)                                        gcAACCTTGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACaa                                >  1:5420151/1‑100 (MQ=255)                                               tGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACACAGCGaga                         >  1:5054270/1‑98 (MQ=255)                                               tGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCggag                         >  1:413794/1‑97 (MQ=255)                                               tGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGaaa                         >  1:3369038/1‑100 (MQ=255)                                               tGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGaaa                         >  1:4523065/1‑100 (MQ=255)                                                ggCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGaaag                        >  1:434581/1‑99 (MQ=255)                                                              aCAGAATTAAAGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGAAACATTGGAAGttgttg          >  1:3365315/1‑100 (MQ=255)                                                                      aaaGTTGTCACAAGATGATATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGAAACATTGGAAGTTGTTGTAAAgggc  >  1:2744982/1‑99 (MQ=255)                                                                                        |                                                                                  ATCACATCGTGAATTAGATCAAGAAAAATCCTCAATCGCGCAACCTTGGCATAAACATGAAACAGAATTAAAGTTGTCACAAGATGAGATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGAAACATTGGAAGTTGTTGTAAAGGGT  >  NC_007793/2733235‑2733403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑