Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	502,496	G→A	C193Y (TGT→TAT)	SAUSA300_RS02385 →	glutamate synthase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	502,496	0	G	A	95.5%	51.6 / ‑6.1	22	C193Y (TGT→TAT)	SAUSA300_RS02385	glutamate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/1);  minor base T (1/0);  total (21/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTGCTGAAGAACTTAATCTACTAGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTGTGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCATTACTTTCATTAATGGTGTTG  >  NC_007793/502415‑502588                                                                                  |                                                                                             ctgctgctgAAGAACTTAATCTACTAGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGaa                                                                            >  1:500680/1‑100 (MQ=255)   gctgctgAAGAACTTAATCTACTAGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAAc                                                                          >  1:5633250/1‑100 (MQ=255)   gctgctgAAGAACTTAATCTACTAGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAAc                                                                          >  1:3002986/1‑100 (MQ=255)                  aaTCTACTAGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTgg                                                           >  1:5109248/1‑100 (MQ=255)                       actaGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGa                                                      >  1:1631047/1‑100 (MQ=255)                       actaGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGa                                                      >  1:1001024/1‑100 (MQ=255)                       actaGGATATCAAGTAACTATTTATGAACGTGCTAGAGAAACAGGCGGTTTATTATTGTTTGGTATTCCGATTTTGAAACTTGATAAAGATGTGGTTCGa                                                      >  1:5268107/1‑100 (MQ=255)                        ctaGGATATCAAGTAACTATTTATGAACGTGCTAGCGAATCAGGCGGCTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGCTGTGGTTCGAc                                                     >  1:700357/1‑100 (MQ=255)                        agtagATATCAAGTAACTATTTCTGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGAc                                                     >  1:624398/5‑100 (MQ=255)                          aGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGGATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGAcgt                                                   >  1:6293643/1‑100 (MQ=255)                                  aaGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTaa                                           >  1:3767391/1‑100 (MQ=255)                                  aaGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTaa                                           >  1:2700562/1‑100 (MQ=255)                                       aCTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTaa                                      >  1:1516834/1‑100 (MQ=255)                                              aTGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGgaaga                               >  1:2940895/1‑100 (MQ=255)                                                   cGTGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCgg                          >  1:5103517/1‑100 (MQ=255)                                                    atGCTAGAGAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCggg                         >  1:4010855/2‑100 (MQ=255)                                                         agagAATCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGCATTAAGTTAATGGAAGAAGCGGGCATTa                    >  1:180358/1‑100 (MQ=255)                                                              aTCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCagatcggaa               >  1:3776171/1‑92 (MQ=255)                                                              aTCAGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCAtagatcgg               >  1:5583426/1‑93 (MQ=255)                                                                  ggCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCATTACTTTCATTa           >  1:5540364/1‑100 (MQ=255)                                                                         tattaATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCATTACTTTCATTAATGgtgt    >  1:4355761/1‑100 (MQ=255)                                                                           ttaATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCATTACTTTCATTAATGgtgtat  <  1:1489818/100‑3 (MQ=255)                                                                                  |                                                                                             CTGCTGCTGAAGAACTTAATCTACTAGGATATCAAGTAACTATTTATGAACGTGCTAGAGAATCAGGCGGTTTATTAATGTGTGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAGAAGCGGGCATTACTTTCATTAATGGTGTTG  >  NC_007793/502415‑502588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑