Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,717,834	C→T	W26* (TGG→TGA)	SAUSA300_RS13950 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,717,834	0	C	T	100.0%	31.0 / NA	13	W26* (TGG→TGA)	SAUSA300_RS13950	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/13);  total (0/13)

TATCACTCAGATCACCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATACCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCT  >  NC_007793/2717740‑2717891                                                                                               |                                                          tATCACTCAGATCACCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATAtcatcc                                                      <  1:6510361/100‑1 (MQ=255)        cAGATCACCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCctca                                               <  1:2650063/100‑2 (MQ=255)                cTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACtt                                       <  1:349416/100‑2 (MQ=255)                         ttGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAAc                              <  1:4691765/100‑1 (MQ=255)                                       tGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTgag                <  1:3785635/100‑3 (MQ=255)                                           cTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTccc            <  1:5234447/100‑1 (MQ=255)                                            tATGTCTGAAAGTTCACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCt           <  1:4924574/100‑1 (MQ=255)                                              tGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTga         <  1:1508860/100‑2 (MQ=255)                                              tGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGt         <  1:1251715/100‑1 (MQ=255)                                              tGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGt         <  1:2857014/100‑1 (MQ=255)                                                 cTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTGgc      <  1:2228001/100‑1 (MQ=255)                                                  tGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTgct     <  1:5798595/100‑2 (MQ=255)                                                  tGAAAGTTTACGATCATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTgcc     <  1:4907898/100‑2 (MQ=255)                                                     aaGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATATCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCt  <  1:4332394/100‑1 (MQ=255)                                                                                               |                                                          TATCACTCAGATCACCTTCAACGATTGATAAATGCGGATGTCCTATGTCTGAAAGTTTACGATTATTCTTATTTCTAGTTAATGCACTTACATACCATCCATCCTCTAACAACTGTTTTACAACTGCATTACCTTGCTTCCCTGTTGCGCCT  >  NC_007793/2717740‑2717891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑