Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,709,872	T→C	L303L (TTA→TTG)	SAUSA300_RS13905 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,709,872	0	T	C	100.0%	98.2 / NA	37	L303L (TTA→TTG)	SAUSA300_RS13905	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/31);  total (6/31)

ATACCGGTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGTAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATGAATAAAGTTGCAAATGATATAATGTAA  >  NC_007793/2709775‑2709962                                                                                                  |                                                                                           ataCCGGTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCaa                                                                                          <  1:6435238/100‑1 (MQ=255) ataCCGGTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCaa                                                                                          >  1:6495884/1‑100 (MQ=255) ataCCGGTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCaa                                                                                          <  1:715120/100‑1 (MQ=255) ataCCGGTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCaa                                                                                          <  1:715837/100‑1 (MQ=255)   aCCGGTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCaagt                                                                                        <  1:6401860/100‑3 (MQ=255)    ttccgatctGTCCTCGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAgg                                                                                       <  1:4651758/92‑1 (MQ=255)      ggTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGtt                                                                                     <  1:2931828/100‑1 (MQ=255)         aTTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCa                                                                                  <  1:3567788/100‑1 (MQ=255)          tttGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCAt                                                                                 <  1:1104501/100‑1 (MQ=255)              cccTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACtt                                                                             >  1:165566/2‑100 (MQ=255)                       gTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTa                                                                    >  1:5808546/1‑100 (MQ=255)                         aataaCTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGc                                                                  >  1:3424339/1‑100 (MQ=255)                         aataaCTGGTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGc                                                                  <  1:5270808/100‑1 (MQ=255)                             aCTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTGCATACTTAAAAAGTTAGCAAAt                                                              <  1:1466830/100‑1 (MQ=255)                                  tCACAAAAACAGTGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACt                                                         <  1:884343/100‑1 (MQ=255)                                  tCACAAAAACAATGATAGTATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACt                                                         <  1:4957361/100‑1 (MQ=255)                                  tCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACt                                                         <  1:501644/100‑1 (MQ=255)                                  tCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACt                                                         <  1:4924384/100‑1 (MQ=255)                                  tCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACt                                                         <  1:5387940/100‑1 (MQ=255)                                  tCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACt                                                         <  1:3348849/100‑1 (MQ=255)                                  tCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACt                                                         <  1:708695/100‑1 (MQ=255)                                    acaAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAg                                                       <  1:1695887/100‑1 (MQ=255)                                    acaAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAg                                                       <  1:1433583/100‑1 (MQ=255)                                     caAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTGGa                                                      <  1:1766197/100‑1 (MQ=255)                                          aCAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGAt                                                 <  1:5772911/100‑1 (MQ=255)                                             aTGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGtg                                              <  1:1349734/100‑2 (MQ=255)                                             aTGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTa                                              <  1:3189484/100‑1 (MQ=255)                                              tGATAGGATAATGCTATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTAc                                             <  1:4885940/100‑1 (MQ=255)                                               gATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTAcg                                            <  1:520117/100‑2 (MQ=255)                                                        aTGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCa                                   <  1:1647123/100‑1 (MQ=255)                                                              tAAGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATg                             <  1:5850282/100‑1 (MQ=255)                                                              caaGTATAAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATg                             >  1:2969102/2‑100 (MQ=255)                                                                  tataAGCTATATGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCaataacggctg                         <  1:5825179/100‑7 (MQ=255)                                                                          tccgatctgTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATGAATAAAGTTgag                 <  1:505853/92‑3 (MQ=255)                                                                           tatGATCGTTTACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATGAATAAAGTTGCaa                <  1:1632417/100‑1 (MQ=255)                                                                                   tttACCTATAATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATGAATAAAGTTGCAAATGAtata        >  1:3943487/1‑100 (MQ=255)                                                                                         tataATTAGCAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATGAATAAAGTTGCAAATGATATAATGTaa  <  1:2373184/100‑1 (MQ=255)                                                                                                  |                                                                                           ATACCGGTATTTGTCCTTGTACGTAATAACTGTTCACAAAAACAATGATAGGATAATGCCATAAGTATAAGCTATATGATCGTTTACCTATAATTAGTAAAGGTTTCATACTTAAAAATTTAGCAAATAAACTAGAAGGATGTACCGCAATTGCAATAATGAATAAAGTTGCAAATGATATAATGTAA  >  NC_007793/2709775‑2709962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑