Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,708,564	T→G	A15A (GCA→GCC)	SAUSA300_RS13900 ←	CHAP domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,708,564	0	T	G	100.0%	49.5 / NA	20	A15A (GCA→GCC)	SAUSA300_RS13900	CHAP domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/18);  total (2/18)

TTGTATACGTATAGCTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTTGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCAATAATCAAAACACTCTATACATATAGTCATTA  >  NC_007793/2708467‑2708657                                                                                                  |                                                                                               ttGTATACGTATAGTTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGc                                                                                              <  1:4859961/100‑1 (MQ=255)  tGTATACGTATAGCTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCa                                                                                             <  1:2209233/100‑1 (MQ=255)  tGTATACGTATAGCTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCa                                                                                             <  1:3617985/100‑1 (MQ=255)            taGCTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCctgcaa                                                                                   <  1:3449894/100‑3 (MQ=255)             aGCTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGt                                                                                  <  1:4677481/100‑1 (MQ=255)              gCTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTa                                                                                 >  1:5579937/1‑100 (MQ=255)               cTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAg                                                                                <  1:329508/100‑1 (MQ=255)                                              cATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGTAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATaaa                                                <  1:4197540/100‑2 (MQ=255)                                                             cTGCGGCCTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑‑TTTTTTCATAATTAAATATCCTCCCaat                                <  1:1303025/100‑1 (MQ=255)                                                              tGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCaat                                <  1:225028/100‑1 (MQ=255)                                                               gcgtcctgtgCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTATCGACTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCaata                               <  1:6506140/94‑1 (MQ=255)                                                               gCGTCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCaata                               >  1:990065/1‑100 (MQ=255)                                                               gCGGCCTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCaata                               <  1:2985221/100‑1 (MQ=255)                                                                cGGCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGATTTTTTTTCATAATTAAATATCCTCCCaata                               <  1:3322070/100‑1 (MQ=255)                                                                  gCTTGTGCATCATGTCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCAATAATc                            <  1:201233/100‑1 (MQ=255)                                                                                tCCTGCAAATGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCAATAATCAAAACACTCTATAc              <  1:3790609/100‑1 (MQ=255)                                                                                  cTGCAAAAGCGATAGTGGCAAGTCCTGCTGTAGAGATTGGAGCTGTAACGA‑AAAAAATCATAATTAAATAGCATAAAAATAATCAAAACACTCTATACag            <  1:4785677/100‑2 (MQ=255)                                                                                    tgctgtGCGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCAATAATCAAAACACTCTATACatat          <  1:605274/95‑1 (MQ=255)                                                                                           cGATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCAATAATCAAAACACTCTATACATATAGTCAtt   <  1:1034143/100‑1 (MQ=255)                                                                                            gATAGTGGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCAATAATCGAAACACTCTATACATATAGTCATTa  <  1:2593672/100‑1 (MQ=255)                                                                                                  |                                                                                               TTGTATACGTATAGCTGTATGATTGAGCGTCATTAGAATTATATCCATTGTTATTTTGTTCTGCGGCTTGTGCATCATGTCCTGCAAATGCGATAGTTGCAAGTCCTGCTGTAGCGATTGTAGCTGTAACGA‑TTTTTTTCATAATTAAATATCCTCCCAATAATCAAAACACTCTATACATATAGTCATTA  >  NC_007793/2708467‑2708657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑