Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	501,449	G→A	V1350I (GTC→ATC)	SAUSA300_RS02380 →	glutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	501,449	0	G	A	100.0%	66.4 / NA	27	V1350I (GTC→ATC)	SAUSA300_RS02380	glutamate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/1);  total (26/1)

CATTCTATGGTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTGTCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAACTTCGGTCAAGGTATG  >  NC_007793/501363‑501544                                                                                       |                                                                                                cATTCTATGGTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGACAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTAt                                                                                    >  1:3821005/1‑100 (MQ=255)       aTGGTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGa                                                                              >  1:4313497/1‑100 (MQ=255)       aTGGTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGa                                                                              >  1:4031087/1‑100 (MQ=255)        cagTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGAc                                                                             >  1:5798603/3‑100 (MQ=255)         cgTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGAca                                                                            >  1:2538755/2‑99 (MQ=255)           cgCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCACGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCAt                                                                          >  1:6128602/2‑100 (MQ=255)            gCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGCAATAGTGGTGTAGATGTTATCGTTCAAGGTACCGGCGagatcg                                                                         >  1:3488736/1‑95 (MQ=255)            gCGACAAGTGGTAAGGCATTTATTAACAGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGATATCGGCGACCATg                                                                         >  1:4982112/1‑100 (MQ=255)                taaGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGAtt                                                                     >  1:573782/2‑100 (MQ=255)                 taGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTa                                                                    >  1:2441047/2‑100 (MQ=255)                       tAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGtat                                                              >  1:755376/1‑100 (MQ=255)                        aaGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGtata                                                             >  1:3274268/1‑100 (MQ=255)                        aaGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGtata                                                             >  1:4213762/1‑100 (MQ=255)                                   taACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGa                                                  >  1:763872/1‑100 (MQ=255)                                         caGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGCATATGACTGGTGGACATGTc                                            >  1:794763/2‑100 (MQ=255)                                          aGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGCTGATATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCa                                           >  1:3465187/1‑100 (MQ=255)                                           ttGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCAt                                          >  1:2593286/2‑100 (MQ=255)                                             gCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGCCTGGTGGACATGTCATTa                                        >  1:791336/1‑100 (MQ=255)                                             gCAGGAGAAAGATTCTGTATTAGAAATAGCGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTa                                        >  1:617650/1‑100 (MQ=255)                                             gCAGGAGAAAGATTCTGTATTAGAAATAGCGGCGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTa                                        >  1:218531/1‑100 (MQ=255)                                                    aaaGATTCTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGTTTAGAGTATATGACTGGTGGACATGTCATTAATTTAgg                                 >  1:2785375/1‑100 (MQ=255)                                                           cTGTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGaagat                          >  1:2425058/1‑96 (MQ=255)                                                             gTATTAGAAATAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAgg                        >  1:2262397/1‑100 (MQ=255)                                                                ttAGAAATAGTGGTGTAGATTTTATCGTTGAAAGTATCGGCGACCATGGATTAGAGTATATGACTAGTGGACATGTCATTAATTTAGGTGATGTAGGTaa                     <  1:1214842/100‑1 (MQ=255)                                                                    ccaTAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAAc                 >  1:4770763/3‑100 (MQ=255)                                                                       tAGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAACTTc              >  1:652652/1‑100 (MQ=255)                                                                        aGTGGTGTAGATGTTATCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAACTTCg             >  1:2094670/1‑100 (MQ=255)                                                                                   tgttatCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAACTTCGGTCAAGGTATg  <  1:4345029/100‑1 (MQ=255)                                                                                       |                                                                                                CATTCTATGGTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTCTGTATTAGAAATAGTGGTGTAGATGTTGTCGTTGAAGGTATCGGCGACCATGGATTAGAGTATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAACTTCGGTCAAGGTATG  >  NC_007793/501363‑501544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑