Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,686,641	C→A	R483R (CGA→AGA)	SAUSA300_RS13805 →	ATP‑dependent Clp protease ATP‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,686,641	0	C	A	100.0%	73.8 / NA	28	R483R (CGA→AGA)	SAUSA300_RS13805	ATP‑dependent Clp protease ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/3);  total (25/3)

TGGCAGTTTCCTATTTGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTCGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAATTCAAATACG  >  NC_007793/2686544‑2686736                                                                                                  |                                                                                                tGGCAGTTTCCTATTTGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGa                                                                                               >  1:5512949/1‑100 (MQ=255) tGGCAGTTTCCTATTTGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGa                                                                                               >  1:5073408/1‑100 (MQ=255) tGGCAGTTTCCTATTTGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGa                                                                                               >  1:5019398/1‑100 (MQ=255) ctgCAGTTTCCTATTTGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGa                                                                                               >  1:2238415/3‑100 (MQ=255)     ctcttCCGATCTGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCAATGATTAGACTTg                                                                                           <  1:2895966/97‑1 (MQ=255)              tttGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAGCAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTg                                                                                  <  1:6045191/100‑1 (MQ=255)                 gttgGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAat                                                                               >  1:6449874/1‑100 (MQ=255)                  ttgGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAata                                                                              >  1:57214/1‑100 (MQ=255)                  ctgGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAata                                                                              >  1:4150346/2‑100 (MQ=255)                   tgGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAatat                                                                             >  1:4686710/1‑100 (MQ=255)                    ggCCCTACTGGTGTTGGTAAAGCAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAAtata                                                                            >  1:5714597/1‑100 (MQ=255)                    ggCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAAtata                                                                            >  1:6440724/1‑100 (MQ=255)                    actccTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAAtata                                                                            >  1:4584650/4‑100 (MQ=255)                          aCTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGaaaagatcggaa                                                                      >  1:1994772/1‑90 (MQ=255)                          aCTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAATATAGTGaca                                                                      >  1:2696975/1‑100 (MQ=255)                                 gtGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGTGTGAATATAGTGACACAACAGc                                                               >  1:4169372/2‑100 (MQ=255)                                  tGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCt                                                              >  1:1794764/1‑100 (MQ=255)                                   ggTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGAGTAGACTTGATATGAGTGAATATAGTGACACAACAGCTg                                                             <  1:4467886/100‑1 (MQ=255)                                      aaaaCAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGttt                                                          >  1:1715872/1‑100 (MQ=255)                                                            tAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATAATTGGTACAActgc                                    >  1:4902413/1‑100 (MQ=255)                                                                            tGGTAATAAAGATGCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTat                    >  1:106279/1‑100 (MQ=255)                                                                                      cttGCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAAtt          >  1:5051064/3‑100 (MQ=255)                                                                                       aTGCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAATTc         >  1:5255214/1‑100 (MQ=255)                                                                                         gCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAATTCaa       >  1:3703440/1‑100 (MQ=255)                                                                                         gCACTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAATTCaa       >  1:181486/1‑100 (MQ=255)                                                                                           ccTGATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACGACTGCTGGTTATGTTGGTTATGATGACAATTCAAAt     >  1:1150381/2‑100 (MQ=255)                                                                                              gATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAATTCAAATACg  >  1:5826022/1‑100 (MQ=255)                                                                                              gATTAGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAATTCAAATACg  >  1:6484778/1‑100 (MQ=255)                                                                                                  |                                                                                                TGGCAGTTTCCTATTTGTTGGCCCTACTGGTGTTGGTAAAACAGAGCTTGCTAAACAATTAGCAATTGATTTATTTGGTAATAAAGATGCACTGATTCGACTTGATATGAGTGAATATAGTGACACAACAGCTGTTTCAAAAATGATTGGTACAACTGCTGGTTATGTTGGTTATGATGACAATTCAAATACG  >  NC_007793/2686544‑2686736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑