Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,685,939	T→G	S249A (TCT→GCT)	SAUSA300_RS13805 →	ATP‑dependent Clp protease ATP‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,685,939	0	T	G	100.0%	142.8 / NA	53	S249A (TCT→GCT)	SAUSA300_RS13805	ATP‑dependent Clp protease ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (50/3);  total (50/3)

TATCTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATTCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTCGCGAAA  >  NC_007793/2685844‑2686026                                                                                                |                                                                                        tATCTGATATTTTGAAACCTGCATTACGTCGTGGAGAGATTGCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGAtgctgc                                                                                     >  1:4407641/1‑100 (MQ=255) tATCTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGAtgctgc                                                                                     <  1:4757199/100‑1 (MQ=255)  aTCTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCa                                                                                    >  1:5103373/1‑100 (MQ=255)     tGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACAAAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTa                                                                                 <  1:481294/100‑1 (MQ=255)      gATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTaa                                                                                >  1:3117658/1‑100 (MQ=255)      gATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTaa                                                                                >  1:1311307/1‑100 (MQ=255)      gATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTaa                                                                                >  1:1194529/1‑100 (MQ=255)                 tccTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGAttt                                                                     >  1:4496083/2‑100 (MQ=255)                    tGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAAt                                                                  >  1:1184310/1‑100 (MQ=255)                     gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACgcgctagatcggaa                                                                 >  1:2993819/1‑90 (MQ=255)                     gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                 >  1:1169932/1‑100 (MQ=255)                     gCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATg                                                                 >  1:3689677/1‑100 (MQ=255)                         ccgaacGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGt                                                             >  1:189980/6‑100 (MQ=255)                          tgtgcGTGGTGAGATTTCTATTATTGGTGCGACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCGTTAACGCGCAGATTTAATGAAGTg                                                            >  1:2077602/5‑100 (MQ=255)                          tccgcGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTg                                                            >  1:2657677/5‑100 (MQ=255)                            gccGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                                          >  1:1817403/3‑100 (MQ=255)                            ctCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                                          >  1:5257821/2‑100 (MQ=255)                            accGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCt                                                          >  1:901660/3‑100 (MQ=255)                             ccGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCtt                                                         >  1:2666398/2‑100 (MQ=255)                                 tgtgAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTCGTTa                                                     >  1:5525625/2‑100 (MQ=255)                                 ggtgAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTa                                                     >  1:3225648/1‑100 (MQ=255)                                    gagaTTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATg                                                  >  1:6103407/1‑100 (MQ=255)                                       ttttCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAAc                                               >  1:5582045/2‑100 (MQ=255)                                         ttCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCa                                             <  1:5551937/100‑1 (MQ=255)                                             attattGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAgcg                                         >  1:5824460/1‑100 (MQ=255)                                             attattGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAgcg                                         >  1:6229059/1‑100 (MQ=255)                                                   ggTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAg                                   >  1:1465156/1‑100 (MQ=255)                                                   cctGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAg                                   >  1:3606966/3‑100 (MQ=255)                                                     tGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGAt                                 >  1:2361399/1‑100 (MQ=255)                                                     ggCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGAt                                 >  1:2789510/2‑100 (MQ=255)                                                      gCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa                                >  1:1072978/1‑100 (MQ=255)                                                      gCAACAACAAAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATa                                >  1:1410040/1‑100 (MQ=255)                                                        tacaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACt                              >  1:4544621/2‑100 (MQ=255)                                                        aacaacACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACt                              >  1:4162059/1‑100 (MQ=255)                                                        aacaacACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACt                              >  1:1755640/1‑100 (MQ=255)                                                        aacaacACAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACt                              >  1:4497852/1‑100 (MQ=255)                                                         tttacacAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:3158917/4‑100 (MQ=255)                                                         gcaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:3446012/2‑100 (MQ=255)                                                         ctgacccaAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:1700626/7‑100 (MQ=255)                                                         ccaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:3899351/2‑100 (MQ=255)                                                         acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGTACCAAGCGCTAAAGATACTg                             >  1:4753476/1‑100 (MQ=255)                                                         acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:2454091/1‑100 (MQ=255)                                                         acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:2308050/1‑100 (MQ=255)                                                         acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:4403301/1‑100 (MQ=255)                                                         acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:3929266/1‑100 (MQ=255)                                                         acaacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTg                             >  1:6176690/1‑100 (MQ=255)                                                           aacaCAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGtt                           >  1:3974200/1‑100 (MQ=255)                                                            ccacaAGATGAATATCGAAACAATATTCGTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTg                          >  1:1151355/2‑100 (MQ=255)                                                            acacAAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTg                          >  1:4618449/1‑100 (MQ=255)                                                             cacaAGATGAATATCGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGa                         >  1:4428978/1‑100 (MQ=255)                                                                           cGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAggca           >  1:330870/1‑98 (MQ=255)                                                                           cGAAACAATATTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTa           >  1:1260024/1‑100 (MQ=255)                                                                           cGAAACAATATTCTCAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTa           <  1:4809799/100‑1 (MQ=255)                                                                                    atTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAGTGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTCGCGaaa  >  1:2109642/1‑100 (MQ=255)                                                                                    atTCTTAAAGATGCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTcgcggta  >  1:727530/1‑97 (MQ=255)                                                                                                |                                                                                        TATCTGATATTTTGAAACCTGCATTAAGTCGTGGTGAGATTTCTATTATTGGTGCAACAACACAAGATGAATATCGAAACAATATTCTTAAAGATTCTGCATTAACGCGCAGATTTAATGAAGTGCTTGTTAATGAACCAAGCGCTAAAGATACTGTTGAAATTTTAAAAGGTATTCGCGAAA  >  NC_007793/2685844‑2686026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑