Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,667,711	C→T	G112D (GGC→GAC)	SAUSA300_RS13710 ←	transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,667,711	0	C	T	100.0%	11.6 / NA	6	G112D (GGC→GAC)	SAUSA300_RS13710	transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/6);  total (0/6)

GTCCGAAAGCAATTGCTGGTGCAAATAAGATCACCACAATTAAGTCCAAGCCTTCTGGAACTTTCTTTTCAATCCATTTAATTAAAAAAGCTACGCCATAAGCTGCGATGAATGCTGGTAATAATTTAAAGTCATGTAATACTAAACCAACAATGACCGCAAATACTGGTGCAAC  >  NC_007793/2667616‑2667790                                                                                                |                                                                                gTCCGAAAGCAATTGCTGGTGCAAATAAGATCACCACAATTAAGTCCAAGCATTCTGGAACTTTCTTTTCAATCCATTTAATTAAAAAAGCTACGTCATa                                                                             <  1:2569899/100‑1 (MQ=255)           aaTTGCTGGTGCAAATAAGATCACCACAATTAAGTCCAAGCCTCCTGGAACTTTCTTTTCAATCCATTTAATTAAAAAAGCTACGTCATAAGCTGCGATg                                                                   <  1:4949489/100‑1 (MQ=255)                     gCAAATAAGATCACCACAATTAAGTCCAAGCCTTCTGGAACTTTCTTTTCAATCCATTTAATTAAAAAAGCTACGTCATAAGCTGCGATGAATGCTGGta                                                         <  1:3411999/100‑1 (MQ=255)                                                                          ccATTTAATTAAAAAAGCTACGTCATAAGCTGCGATGAATGCTGGTAATAATTTAAAGTCATGTAATACTAAACCAACAATGACCGCAAATACTGGTGCa    <  1:3640398/100‑1 (MQ=255)                                                                          atctttCATTAAAAAAGCTACGTCATAAGCTGCGATGAATGCTGGTAATAATTTAAAGTCATGTAATACTAAACCAACAATGACCGCAAATACTGGTGCa    <  1:2954373/97‑1 (MQ=255)                                                                            aTTTAATTAAAAAAGCTACGTCATAAGCTGCGATGAATGCTGGTAATAATTTAAAGTCATGTAATACTAAACCAACAATGACCGCAAATACTGGTGCAAc  <  1:2317602/100‑1 (MQ=255)                                                                                                |                                                                                GTCCGAAAGCAATTGCTGGTGCAAATAAGATCACCACAATTAAGTCCAAGCCTTCTGGAACTTTCTTTTCAATCCATTTAATTAAAAAAGCTACGCCATAAGCTGCGATGAATGCTGGTAATAATTTAAAGTCATGTAATACTAAACCAACAATGACCGCAAATACTGGTGCAAC  >  NC_007793/2667616‑2667790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑