Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,652,084	C→T	D267D (GAC→GAT)	SAUSA300_RS13615 →	fructose 1,6‑bisphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,652,084	0	C	T	95.0%	48.7 / NA	20	D267D (GAC→GAT)	SAUSA300_RS13615	fructose 1,6‑bisphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/3);  minor base A (1/0);  total (17/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGGGTTGGAGCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGACGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTATGACGCAGATAATCCTGCTTTTAAGCCTA  >  NC_007793/2651993‑2652163                                                                                            |                                                                                tttGGGTTGGAGCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATga                                                                         >  1:4433336/1‑99 (MQ=255) aatGGGTTGGAGCCTATGCTGGGTCAAAAGGATGCTTAGCAAACTTACTTCGATTTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTACgg                                                                         >  1:704675/3‑100 (MQ=255)   tGGGTTGGAGCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCa                                                                       >  1:5488001/1‑100 (MQ=255)    gggTTGGAGCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCAt                                                                      >  1:4493477/1‑100 (MQ=255)           aGCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTa                                                               >  1:2602235/1‑100 (MQ=255)             ccTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAg                                                             >  1:1659671/1‑100 (MQ=255)                aTGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGAcc                                                          >  1:2498327/1‑100 (MQ=255)                aTGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGAcc                                                          >  1:4343996/1‑100 (MQ=255)                   cTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCAcg                                                       <  1:6229690/100‑2 (MQ=255)                                  tgttAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGa                                        >  1:2088350/3‑100 (MQ=255)                                     tAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGaaaa                                     >  1:6143003/1‑100 (MQ=255)                                        cccagttacttCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATa                                  >  1:5200354/6‑100 (MQ=255)                                           acttacttCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTa                               <  1:1999738/100‑1 (MQ=255)                                                          gtgCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCGCTGCTTACTTTAGCTGAAAAATACTATGACGCAGATAATcc                >  1:5985600/1‑100 (MQ=255)                                                          gtgCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTATGACGCAGAtcggaa                >  1:4674417/1‑95 (MQ=255)                                                          gtgCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTATGACGCAGATAATcc                >  1:6367381/1‑100 (MQ=255)                                                           tgCACGTTATGATAATTTAGTTATTATCGAAGAAGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATATTATGACGCAGATAATCCt               >  1:2075101/1‑100 (MQ=255)                                                            gCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTATGACGCATATAATCCTg              >  1:6077787/1‑100 (MQ=255)                                                            gCACGTTATGATAATTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTATGACACAGAtcggaaga              >  1:6377248/1‑93 (MQ=255)                                                                        aaTTTAGATATTATCGAAGATGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTATGACGCAGATAATCCTGCTTTTAAGCCtg  <  1:3026176/100‑2 (MQ=255)                                                                                            |                                                                                TTTGGGTTGGAGCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGATAATTTAGATATTATCGAAGACGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCTGAAAAATACTATGACGCAGATAATCCTGCTTTTAAGCCTA  >  NC_007793/2651993‑2652163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑