Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,624,923	C→A	R443L (CGG→CTG)	SAUSA300_RS13505 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,624,923	0	C	A	100.0%	35.2 / NA	15	R443L (CGG→CTG)	SAUSA300_RS13505	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/15);  total (0/15)

TTTTTGTCCTTCTCTTGTTACTTTTTCTGTCCCTGGTGCTAAATCCGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAAC‑TTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGGTGTAAA  >  NC_007793/2624878‑2625008                                              |                                                                                       tttttGTCCTTCTCTTGTTACTTTTTCTGTCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAAC‑ttttttttctaag                                 <  1:2418159/100‑7 (MQ=255)      tccgatctcTTGCTACTTTTTCTGTCCCTGGTACTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGt                             <  1:4267691/95‑1 (MQ=17)               ttGTTACTTTTTCTGTCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGtttt                    <  1:605307/100‑1 (MQ=255)                   tattttttCTGTCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGgc                <  1:5574732/97‑1 (MQ=18)                   tACTTTTTCTGTCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAAC‑TTTTTTTCTAATTCTGTTATTGTTTTTGgcg               <  1:1865089/100‑1 (MQ=255)                    tcTTTTTCTGTCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGgcg               <  1:2795328/99‑1 (MQ=255)                             gTCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAAC‑TTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTTTGgtgt     <  1:4719307/100‑1 (MQ=255)                              tCCCTGGTGCTAGATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGgtgt     <  1:4604127/100‑1 (MQ=25)                              tCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGGATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGgtgt     <  1:2593309/100‑1 (MQ=25)                              tCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGgtgg     <  1:2030566/100‑2 (MQ=255)                              tCCCTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCACTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGgtgt     <  1:894736/100‑1 (MQ=25)                              tCCCTGGTGCTAAATCAGGATTAAATTTACGTTCCTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGgtgt     <  1:90405/100‑1 (MQ=25)                               cccTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGGTGTa    <  1:3606107/100‑1 (MQ=255)                                ccTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAGCTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGGTGTaa   <  1:681245/100‑1 (MQ=25)                                 cTGGTGCTAAATCAGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAACTTTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGGTGTaaa  <  1:5295579/100‑1 (MQ=255)                                              |                                                                                       TTTTTGTCCTTCTCTTGTTACTTTTTCTGTCCCTGGTGCTAAATCCGGATTAAATTTACGTTCTTTCTTGAATGGAATCTCTTCAAC‑TTTTTTTCTAATTCTGTTATTGTTTTTGGCGCTTCTGGTGTAAA  >  NC_007793/2624878‑2625008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑