Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,616,847	T→C	I280V (ATT→GTT)	SAUSA300_RS13465 ←	helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,616,847	0	T	C	91.9%	186.9 / 4.0	86	I280V (ATT→GTT)	SAUSA300_RS13465	helicase
Reads supporting (aligned to +/- strand):  ref base T (0/7);  new base C (4/75);  total (4/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

ATCTCT‑ATGTTTTCCTGTCAACAATCCAAAATCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAATCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAGAT  >  NC_007793/2616758‑2616941                                                                                           |                                                                                               aTCTCTAATGTTTTCCTGTCAACAATCCAAAATCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCAtt                                                                                       <  1:893579/100‑1 (MQ=255)     ct‑ATGTTTTCCTGTCAACAATCCAAAATCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGaa                                                                                  >  1:597507/1‑100 (MQ=255)     ct‑ATGTTTTCCTGTCAACAATCCAAAATCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGaa                                                                                  >  1:2099744/1‑100 (MQ=255)       tgtGTTTTCCTGTCAACAATCCAAAATCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAAc                                                                                 <  1:3047709/98‑1 (MQ=255)         atctttCCTGTCAACAATCCAAAATCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACaa                                                                               <  1:4752715/97‑1 (MQ=255)                              aaaTCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGt                                                          >  1:810347/1‑100 (MQ=255)                               aaTCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGtt                                                         <  1:3891356/100‑1 (MQ=255)                                              ttATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACAtg                                          <  1:6312670/100‑2 (MQ=255)                                               tataGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATca                                         <  1:2660511/100‑2 (MQ=255)                                                ataGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCtg                                        <  1:3394179/100‑2 (MQ=255)                                                 taGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTag                                       <  1:2326771/100‑2 (MQ=255)                                                    gTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGc                                    <  1:3603292/100‑1 (MQ=255)                                                       aTACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGcacc                                 <  1:2487556/100‑2 (MQ=255)                                                        tACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACAt                                <  1:2279116/100‑1 (MQ=255)                                                         aCTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATa                               <  1:4418315/100‑1 (MQ=255)                                                         aCTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATa                               <  1:2996154/100‑1 (MQ=255)                                                         aCTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATa                               <  1:108878/100‑1 (MQ=255)                                                         aCTTTTTTAAATTCTTCCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATa                               <  1:6374117/100‑1 (MQ=255)                                                                        tcCTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTg                <  1:6289079/100‑1 (MQ=255)                                                                        cgatctgCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTg                <  1:869937/94‑1 (MQ=255)                                                                        cgatctgCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTg                <  1:2856900/94‑1 (MQ=255)                                                                         tccgatctctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTgg               <  1:3060393/94‑2 (MQ=255)                                                                         ccTTAGCTCTATTTAAGACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGt               <  1:3146995/100‑1 (MQ=255)                                                                         ccTTAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTga               <  1:5649588/100‑2 (MQ=255)                                                                           tctgCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTat             <  1:5084114/97‑2 (MQ=255)                                                                            tggCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:1132484/98‑1 (MQ=255)                                                                            tAGCTTTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:3337678/100‑1 (MQ=255)                                                                            tAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:1914145/100‑1 (MQ=255)                                                                            tAGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:218283/100‑1 (MQ=255)                                                                            ctgCTTTATTTAAAGCCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:5079946/98‑1 (MQ=255)                                                                            ctgCTTTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:5024916/98‑1 (MQ=255)                                                                            ctgCTCTGTTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:1624287/98‑1 (MQ=255)                                                                            ctgCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:2841370/98‑1 (MQ=255)                                                                            ctgCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:664300/98‑1 (MQ=255)                                                                            ctgCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:697965/98‑1 (MQ=255)                                                                            ctgCTCTATTTAAAACCCCCCCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:4177101/98‑1 (MQ=255)                                                                            cgatctcttttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:2977413/92‑1 (MQ=255)                                                                            atctgccaTTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTAcc            <  1:1090925/93‑1 (MQ=255)                                                                             tgCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCa           <  1:4081643/99‑1 (MQ=255)                                                                             aGCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCa           >  1:1432649/1‑100 (MQ=255)                                                                              tctctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAg          <  1:1271919/99‑1 (MQ=255)                                                                              gCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAg          <  1:5276674/100‑1 (MQ=255)                                                                              gCTCTATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAg          <  1:5951639/100‑1 (MQ=255)                                                                               ctctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGt         <  1:5177251/100‑1 (MQ=255)                                                                               ctctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGt         <  1:1504512/100‑1 (MQ=255)                                                                               ctctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATGTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGt         <  1:5620047/100‑1 (MQ=255)                                                                                tctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGtt        <  1:3205032/100‑1 (MQ=255)                                                                                tctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGtt        <  1:2233494/100‑1 (MQ=255)                                                                                 ctgtttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTg       <  1:5058452/97‑1 (MQ=255)                                                                                 ctATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTg       <  1:1519863/100‑1 (MQ=255)                                                                                  ttccgatcttCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:462785/91‑1 (MQ=255)                                                                                  tgtttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAAGCGTTTTACCTGTACCAGTTGc      <  1:6629147/98‑1 (MQ=255)                                                                                  tctttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:4001114/98‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:2115791/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:69771/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:5584779/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:5512338/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:120708/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:5201940/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:5144467/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:3215668/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:2252190/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:3689222/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:2642537/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:3881265/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:3169137/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:2941445/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:4222609/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:424712/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCGCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:66929/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCTCATTATGAACAATAAATAAAAAGTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:4115424/100‑1 (MQ=255)                                                                                  tATTTAAAACCCCCCCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:4804809/100‑1 (MQ=255)                                                                                  cttccgatctCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAGCATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:3709867/91‑1 (MQ=255)                                                                                  cttccgatctCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:980104/91‑1 (MQ=255)                                                                                  cttccgatctCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:2745548/91‑1 (MQ=255)                                                                                  cttccgatctCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:5374127/91‑1 (MQ=255)                                                                                  ctcttccgaTCTCCTCGTTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:6593295/92‑1 (MQ=255)                                                                                  ctcttccgaTCTCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTGACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:886870/92‑1 (MQ=255)                                                                                  ctcttccgaTCTCCTCACTATGAACAATAAATCAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAAACGTTTTACCTGTACCAGTTGc      <  1:3194732/92‑1 (MQ=255)                                                                                  ctcttccgaTCTCCTCACTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:4418011/92‑1 (MQ=255)                                                                                  ctcttccgaTCTCCTCACTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:6333666/92‑1 (MQ=255)                                                                                  ctcttccgaTCTCCCCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGc      <  1:3685560/92‑1 (MQ=255)                                                                                   aTTTAAAACCCCCTTATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGcc     <  1:4962799/100‑2 (MQ=255)                                                                                    tttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAg    <  1:2633606/100‑1 (MQ=255)                                                                                    tttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAg    <  1:5143787/100‑1 (MQ=255)                                                                                    tttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAg    <  1:3715816/100‑1 (MQ=255)                                                                                    tttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAg    <  1:542178/100‑1 (MQ=255)                                                                                    tccgatctCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTGACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAg    <  1:2722142/93‑1 (MQ=255)                                                                                    tccgatctCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAg    <  1:2242545/93‑1 (MQ=255)                                                                                    cttccgaTCTCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAg    <  1:1146025/94‑1 (MQ=255)                                                                                     ttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATTCACATAAAAGCGTTTTACCTGTACCAGTTGccac   <  1:6558578/100‑4 (MQ=255)                                                                                     ttAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAgc   <  1:1515114/100‑2 (MQ=255)                                                                                      tAAAACCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAGag  <  1:5108443/100‑2 (MQ=255)                                                                                           |                                                                                               ATCTCT‑ATGTTTTCCTGTCAACAATCCAAAATCACTATCATTTTTTATAGGTAATACTTTTTTAAATTCTTCCTTAGCTCTATTTAAAATCCCCTCATTATGAACAATAAATAAAAATTTATTAGGGTTTACTTCTCTAACATCTAATGCACATAAAATCGTTTTACCTGTACCAGTTGCAGAT  >  NC_007793/2616758‑2616941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑