Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,610,839	T→C	intergenic (‑464/+72)	SAUSA300_RS13430 ← / ← SAUSA300_RS13435	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,610,839	0	T	C	100.0%	28.3 / NA	13	intergenic (‑464/+72)	SAUSA300_RS13430/SAUSA300_RS13435	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/10);  total (3/10)

TGACATAGATCCCGAAAAATAATGGCTAATAGCCACTTATAGTCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTAATACCTTTTTATACTTTAATATCTTGAATTTT  >  NC_007793/2610797‑2610934                                           |                                                                                                tGACATAGATCCCGAAAAATAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTgtgt                                        <  1:1861119/100‑1 (MQ=255)  gaaaTAGATCCAGAAAAATAATGGCTAATAGCCGCTTATAGCCGAAATGATGAACTATATAATTCATTAATTATTAAATGGATTATATACTTATTGTGtt                                       <  1:5469947/97‑1 (MQ=255)  gACATAGATCCCGAAAAATAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGtt                                       <  1:2797298/100‑1 (MQ=255)  gACATAGATCCCGAAAAATAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGtt                                       <  1:5156849/100‑1 (MQ=255)  gACATAGATCCCGAAAAATAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGtt                                       >  1:6500061/1‑100 (MQ=255)  gACATAGATCCCGAAAAATAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGtt                                       <  1:959711/100‑1 (MQ=255)       aGATCCCGAAAAATAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTa                                  >  1:3935273/1‑100 (MQ=255)                aaaaTAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTAATACCtttt                         <  1:6171936/100‑1 (MQ=255)                 aaaTAATGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTAATACCttttt                        <  1:277394/100‑1 (MQ=255)                    taatGGCTAATAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTAATACCTTTTtatc                     <  1:2934959/100‑2 (MQ=255)                             aTAGCCACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTAATACCTTTTTATACTTTAatag            <  1:1032257/100‑2 (MQ=255)                                 ccACTTATAGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACATATTGTGTTTCTTAATACCTTTTTATACTTTAATATCTTg        <  1:6270282/100‑1 (MQ=255)                                       ataGCCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTAATACCTTTTTATACTTTAATATCTTGAAtttt  >  1:2820239/1‑100 (MQ=255)                                           |                                                                                                TGACATAGATCCCGAAAAATAATGGCTAATAGCCACTTATAGTCGAAATTATGAACTATATAATTCATTTATTATTAAATGGATTATATACTTATTGTGTTTCTTAATACCTTTTTATACTTTAATATCTTGAATTTT  >  NC_007793/2610797‑2610934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑