Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,590,666	A→G	T154A (ACT→GCT)	SAUSA300_RS13320 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,590,666	0	A	G	100.0%	103.4 / NA	39	T154A (ACT→GCT)	SAUSA300_RS13320	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/8);  total (31/8)

AAATCATTTCAATATTTTCGCACTAAATGTAGATTCCTTTACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAACTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGATTAATACCTTTAA  >  NC_007793/2590572‑2590762                                                                                               |                                                                                                 aaaTCATTTCAATATTTTCGCACTAAATGTAGATTCCTTTACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCtata                                                                                             >  1:892324/1‑100 (MQ=255)                        accctGTAGATTCCTTTACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTaaa                                                                      >  1:2019005/5‑100 (MQ=255)                         aaaTGTAGATTCCTTTACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAAt                                                                     >  1:5178080/1‑100 (MQ=255)                                gATTCCTTTACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGa                                                              >  1:4979805/1‑100 (MQ=255)                                       ttACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCa                                                       <  1:6221253/100‑1 (MQ=255)                                       ttACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCa                                                       >  1:1980849/1‑100 (MQ=255)                                        tACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCaa                                                      >  1:108079/1‑100 (MQ=255)                                        tACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCaa                                                      >  1:2134107/1‑100 (MQ=255)                                              cTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtt                                                >  1:6001991/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTctgtagatcgg                                              >  1:5336748/1‑90 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:1765270/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:5954258/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:5198370/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:4288148/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:3201548/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:3130558/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:2823586/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtttt                                              >  1:3007290/1‑100 (MQ=255)                                                ttGACATTTACCCGCTCATTAAAGGGCATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTAtctt                                              >  1:2303840/1‑100 (MQ=255)                                                 tGACATTTACCCGCTCATTACAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTc                                             >  1:2263927/1‑100 (MQ=255)                                                     aTTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATGCTTTGATTTGTCAACTTATTTTCCGTc                                         >  1:4284863/1‑100 (MQ=255)                                                       ttACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCat                                       <  1:1429453/100‑1 (MQ=255)                                                         aCCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCatat                                     >  1:1058412/1‑100 (MQ=255)                                                         aCCCGCTCATTAAAGGGTAAGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCatat                                     <  1:2622597/100‑1 (MQ=255)                                                           tgccTCATTAAAGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATAttt                                   >  1:4268896/4‑100 (MQ=255)                                                                cATTAAAGGGTATGGTTCCATCAGCTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAAtt                              >  1:2466529/1‑100 (MQ=255)                                                                    ctaGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAg                          >  1:6395488/3‑100 (MQ=255)                                                                     aaGGGTATGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGa                         >  1:5216780/1‑100 (MQ=255)                                                                           aTGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTagg                   >  1:4095804/1‑98 (MQ=255)                                                                            tGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGc                  <  1:3970335/100‑1 (MQ=255)                                                                            tGGTTCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGc                  <  1:5544190/100‑1 (MQ=255)                                                                            cttccgatctaGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGc                  <  1:1514093/90‑1 (MQ=255)                                                                               acccATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGa               >  1:5612683/3‑100 (MQ=255)                                                                                tCCATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAAATAAGCAGAt              >  1:4009086/1‑100 (MQ=255)                                                                                 ccATCAGTTTAATAGCTATATTTTCGTTGACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGAtt             <  1:6209023/100‑1 (MQ=255)                                                                                 ccATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGAtt             <  1:1944885/100‑1 (MQ=255)                                                                                 ccATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGAtc             >  1:6378598/1‑99 (MQ=255)                                                                                 ccATCAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAA‑TAAGCAGAtcg            >  1:6520920/1‑98 (MQ=255)                                                                                     cAGTTTAATAGCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAA‑‑AAGCAGAtcggaaga       >  1:3300695/1‑93 (MQ=255)                                                                                          taatagCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGATTAATACCttt    <  1:6431132/100‑1 (MQ=255)                                                                                            atagCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGATTAATACCTTTaa  <  1:2229235/100‑1 (MQ=255)                                                                                            atagCTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGATTAATACCTTTaa  <  1:1728727/100‑1 (MQ=255)                                                                                               |                                                                                                 AAATCATTTCAATATTTTCGCACTAAATGTAGATTCCTTTACTGTCTTTGACATTTACCCGCTCATTAAAGGGTATGGTTCCATCAGTTTAATAACTATATTTTCGTTAACCATTATATTAAATACTTTGATTTGTCAACTTATTTTCCGTCATATTTTAATTCAAGAATTAAGCAGATTAATACCTTTAA  >  NC_007793/2590572‑2590762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑