Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,576,724	T→G	I136L (ATA→CTA)	SAUSA300_RS13245 ←	glycine/betaine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,576,724	0	T	G	100.0%	23.7 / NA	11	I136L (ATA→CTA)	SAUSA300_RS13245	glycine/betaine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (1/10);  total (1/10)

TGCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATATTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCATT  >  NC_007793/2576635‑2576816                                                                                          |                                                                                             agCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGa                                                                                    >  1:123136/2‑100 (MQ=255)  gCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGAt                                                                                   <  1:5248768/100‑1 (MQ=255)   cACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATa                                                                                  <  1:2948381/100‑1 (MQ=255)                                  ttGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCcact                                                   <  1:2851153/100‑2 (MQ=255)                                                    tctTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCttt                                 <  1:5710264/100‑1 (MQ=255)                                                     ctTACAACACCGATACGTTGTTGTTGCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTg                                <  1:6476874/100‑1 (MQ=255)                                                                           gttgCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCGTTTGCACGTTTACCCTTTTCCTCttt          <  1:3323425/100‑1 (MQ=255)                                                                           gttgCCCACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCttt          <  1:921254/100‑1 (MQ=255)                                                                                ccACCTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTcc     <  1:1198543/100‑1 (MQ=255)                                                                                   tcTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCAtt  <  1:726898/99‑1 (MQ=255)                                                                                   ccTGATAGTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCAtt  <  1:1285101/100‑1 (MQ=255)                                                                                          |                                                                                             TGCACCAAAAGGTTCATCCATTAAAATAATATCTTGTTCGGCCGCAAGTGCTCTTACAACACCGATACGTTGTTGTTGCCCACCTGATATTTCTGCTGGATAACGCTCTAAAAATGACTCCGGTAAATCCACAAGTTTAATTAATTCCTTTGCACGTTTATCCTTTTCCTCTTTAGTCCATT  >  NC_007793/2576635‑2576816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑