Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,576,289	C→T	A281T (GCT→ACT)	SAUSA300_RS13245 ←	glycine/betaine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,576,289	0	C	T	100.0%	58.4 / NA	23	A281T (GCT→ACT)	SAUSA300_RS13245	glycine/betaine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/23);  total (0/23)

CCCTGATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGCATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGTTTAATCATTACACCTTCTACAGTCTT  >  NC_007793/2576222‑2576385                                                                    |                                                                                                 cccTGATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACgg                                                                  <  1:4066794/100‑1 (MQ=255)    cgATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGcag                                                               <  1:257589/99‑3 (MQ=255)     gATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGtg                                                              <  1:6092842/100‑2 (MQ=255)      aTTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGtgt                                                             <  1:6019498/100‑3 (MQ=255)            atCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAgg                                                       <  1:5168376/100‑2 (MQ=255)              cTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAgtgt                                                     <  1:1301905/100‑1 (MQ=255)              cTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAgtgt                                                     <  1:424325/100‑1 (MQ=255)              cTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAggag                                                     <  1:1149952/100‑4 (MQ=255)               ttCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGtt                                                    <  1:204280/100‑1 (MQ=255)                 cAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGc                                                  <  1:3258781/100‑1 (MQ=255)                  aaTGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCt                                                 <  1:3810513/100‑1 (MQ=255)                  aaTGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCt                                                 <  1:797446/100‑1 (MQ=255)                   aTGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCtt                                                <  1:5995984/100‑1 (MQ=255)                   aTGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCta                                                <  1:4628215/100‑2 (MQ=255)                         aaGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCtt                                          <  1:2775751/100‑1 (MQ=255)                          aGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTg                                         <  1:354779/100‑1 (MQ=255)                           gAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGt                                        <  1:6324545/100‑1 (MQ=255)                              aCCTAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATc                                     <  1:6252967/100‑1 (MQ=255)                                 tAGTAAATGGTTATTACTATCTACTACAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTg                                  <  1:3551568/100‑1 (MQ=255)                                                           cAAAAATAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGTTTAATCATTACACCTTCTAc        <  1:3776044/100‑1 (MQ=255)                                                             aaaaTAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGATTTAATCATTACACCTTCTACAg      <  1:342586/100‑1 (MQ=255)                                                              aaaTAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGTTTAATCATTACACCTTCTACAGt     <  1:5868964/100‑1 (MQ=255)                                                                 tAGTATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGTTTAATCATTACACCTTCTACAgggcc  <  1:4196551/100‑5 (MQ=255)                                                                    |                                                                                                 CCCTGATTTATATCTTCAATGTCTAAGAAACCTAGTAAATGGTTATTACTATCTACTACAAAAATAGCATCAACACGTTTTTGTCTCATAATATGAACGGCGTCATTCAGTGTTGCTTCTGCTTGTATCGTGATTGGTTTAATCATTACACCTTCTACAGTCTT  >  NC_007793/2576222‑2576385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑