Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,546,294	A→G	Y160H (TAT→CAT)	SAUSA300_RS13090 ←	6‑carboxyhexanoate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,546,294	0	A	G	100.0%	24.2 / NA	11	Y160H (TAT→CAT)	SAUSA300_RS13090	6‑carboxyhexanoate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/10);  total (1/10)

TTAATATCAAATAATCGATGGTAACCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATACGGATGCGCATTGATACAACTAGCAATTGCTAAGGCATCAGTCACTCTTTCATTA  >  NC_007793/2546197‑2546349                                                                                                  |                                                        ttAATATCAAATAATCGATGGTAACCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCg                                                       <  1:6320283/100‑1 (MQ=255)  tAATATCAAATAATCGATGGTGGCCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACGCGCAAAGTTTTCCTTTGACATGCgg                                                      <  1:5144153/100‑1 (MQ=255)  tAATATCAAATAATCGATGGTAACCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCgt                                                      <  1:4669278/100‑2 (MQ=255)  tAATATCAAATAATCGATGGTAACCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCgg                                                      <  1:2807508/100‑1 (MQ=255)    atatCAAATAATCGATGGTAACCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCGGaa                                                    <  1:321250/100‑2 (MQ=255)                        aCCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCGGATGCGCATTGATACAACTAGCa                                <  1:999856/100‑1 (MQ=255)                                tAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGCCATCGGACACGCAAAGTTCTCCTTTGACATGCGGATGCGCATTGATACAACTAGCAATTGTTaa                        >  1:1742179/1‑100 (MQ=255)                                                      cGTATGCGTTAAGTCATCGGACACGCAAAGTTCGCCTTTGACATGCGGATGCGCATTGATACAACGAGCAATTGCTAAGGCATCAGTCACTCTTTCATTa  <  1:2512306/100‑1 (MQ=255)                                                      cGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCGGATGCGCATTGATACAACTAGCAATTGCTAAGGCATCAGTCACTCTTTCATTa  <  1:113642/100‑1 (MQ=255)                                                      cGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCGGATGCGCATTGATACAACTAGCAATTGCTAAGGCATCAGTCACTCTTTCATTa  <  1:3208169/100‑1 (MQ=255)                                                      cGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATGCGGATGCGCATTGATACAACTAGCAATTGCTAAGGCATCAGTCACTCTTTCATTa  <  1:704673/100‑1 (MQ=255)                                                                                                  |                                                        TTAATATCAAATAATCGATGGTAACCAATTTTAGCAGCGGCAAAATAACCTGTCGTATACGTTAAGTCATCGGACACGCAAAGTTCTCCTTTGACATACGGATGCGCATTGATACAACTAGCAATTGCTAAGGCATCAGTCACTCTTTCATTA  >  NC_007793/2546197‑2546349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑