Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,525,762	C→T	E95K (GAA→AAA)	SAUSA300_RS12960 ←	N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,525,762	0	C	T	100.0%	64.4 / NA	25	E95K (GAA→AAA)	SAUSA300_RS12960	N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/21);  total (4/21)

GCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGTAGATA  >  NC_007793/2525665‑2525854                                                                                                  |                                                                                             gCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTAt                                                                                            <  1:646438/100‑1 (MQ=255)     aTGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTc                                                                                        <  1:3944118/100‑1 (MQ=255)      tGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCa                                                                                       <  1:1150846/100‑1 (MQ=255)       gTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAg                                                                                      <  1:5192455/100‑1 (MQ=255)        tACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAgg                                                                                     <  1:4152614/100‑1 (MQ=255)          ctcttccgatcTGCCGCTGACTTTGACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGAt                                                                                   <  1:3198080/90‑1 (MQ=255)                   aCTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCaa                                                                          <  1:1173238/100‑1 (MQ=255)                     tGCCGCTGACTTTAACGTAACTCGCAAAAATGCTTCTTCAACATAAGAAAGTAATAGGTTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAac                                                                        <  1:2179779/100‑1 (MQ=255)                            gACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAgc                                                                 <  1:6508991/100‑2 (MQ=255)                             aCTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGag                                                                <  1:1666793/100‑2 (MQ=255)                                           cGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAGAAGGATAACCCg                                                  <  1:251228/100‑1 (MQ=255)                                           cGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCg                                                  >  1:2730712/1‑100 (MQ=255)                                                                taagtaagTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTcccc                             <  1:754556/100‑1 (MQ=255)                                                                taagtaagTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTcccc                             <  1:1093077/100‑1 (MQ=255)                                                                taagtaagTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTcccc                             <  1:5381777/100‑1 (MQ=255)                                                                taagtaagTAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTcccc                             <  1:3049833/100‑1 (MQ=255)                                                                taagtaagTAATTGGCTACCACAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTcccc                             <  1:5901577/100‑1 (MQ=255)                                                                 aagtaagtAATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCa                            <  1:4664213/100‑1 (MQ=255)                                                                  agtaagtaATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCac                           <  1:4428985/100‑2 (MQ=255)                                                                  agtaagtaATTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCaa                           <  1:6046164/100‑1 (MQ=255)                                                                   gtaagtaaTTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAg                          <  1:3772215/100‑1 (MQ=255)                                                                    taagtaaTTGGCTACCATAGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAgg                         <  1:4230183/100‑1 (MQ=255)                                                                                    aTAGCCTTTACCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATc         >  1:3687815/1‑100 (MQ=255)                                                                                      aGCCTTTCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGt       >  1:1734103/1‑100 (MQ=255)                                                                                           ttCCCTTTATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGTAGata  >  1:3969304/1‑100 (MQ=255)                                                                                                  |                                                                                             GCAGATGTACCTAAAGTAACTGCCGCTGACTTTAACGTATCTCGCAAAAATGCTTCTTCAACATAAGTAAGTAATTGGCTACCATAGCCTTTCCCTTCATACTCAGGATTTGTCGCAAACCACCAGACAAAAGGATAACCCGAAATACTTTTCACACTTCCCCAAGGATATCTAACCGTAATCGTAGATA  >  NC_007793/2525665‑2525854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑