Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,501,150	T→G	intergenic (+1311/‑177)	SAUSA300_RS12850 → / → SAUSA300_RS12855	hypothetical protein/transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,501,150	0	T	G	100.0%	34.9 / NA	15	intergenic (+1311/‑177)	SAUSA300_RS12850/SAUSA300_RS12855	hypothetical protein/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/5);  total (10/5)

TGCACCAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTTCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCTTTTCAAAAATATTCAACTATAAT  >  NC_007793/2501070‑2501241                                                                                 |                                                                                            aaccccAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAg                                                                          >  1:3884168/5‑100 (MQ=255)  gtgccAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGc                                                                         >  1:1115467/4‑100 (MQ=255)   gaCCAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCg                                                                        >  1:442381/2‑100 (MQ=255)   cACCAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCg                                                                        >  1:6155708/1‑100 (MQ=255)    cccAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGt                                                                       >  1:1346906/2‑100 (MQ=255)     ccAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGtt                                                                      >  1:4682237/1‑100 (MQ=255)                           aTCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAAtt                                                >  1:2296515/1‑100 (MQ=255)                                               tcttccgaTCTTTCATATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTa                            <  1:6040075/93‑1 (MQ=255)                                                   ccgaTCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAGAAACAAGCGTTTTCCTAGTAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCtt                        <  1:751553/97‑1 (MQ=255)                                                    cgaTCTTTCTGGTTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCttt                       <  1:573899/98‑1 (MQ=255)                                                      aTCTTTCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCTTTTc                     >  1:3596205/1‑100 (MQ=255)                                                        cTTTCTAATTTTACCTTTATATTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCTTTTCaa                   <  1:4809699/100‑1 (MQ=255)                                                          ttCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTTACTATTACCTTTTCaaaa                 >  1:4453840/1‑100 (MQ=255)                                                          ttCTAATTTTACTTTTATATTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCTTTTCaaaa                 >  1:1874774/1‑100 (MQ=255)                                                                         tataTTGTGCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCTTTTCAAAAATATTCAACTataat  <  1:6481163/100‑1 (MQ=255)                                                                                 |                                                                                            TGCACCAAATAATTTAATTAGACTCTATCGAAAATTTCCAAATTTTGCTTACTATCTTTCTAATTTTACTTTTATATTGTTCGCAATGCATAAAAACAAGCGTTTTCCTAGGAGTTTTCATTAATTTCTTTTATTTTTCACTATTACCTTTTCAAAAATATTCAACTATAAT  >  NC_007793/2501070‑2501241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑