Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,499,398	C→T	intergenic (‑125/‑58)	SAUSA300_RS12845 ← / → SAUSA300_RS12850	PTS sucrose transporter subunit IIBC/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,499,398	0	C	T	100.0%	61.3 / NA	24	intergenic (‑125/‑58)	SAUSA300_RS12845/SAUSA300_RS12850	PTS sucrose transporter subunit IIBC/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/20);  total (4/20)

CCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGCGTGAG  >  NC_007793/2499311‑2499494                                                                                        |                                                                                                 ccACTTTATTGTAATCGGGTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTTCACCATGTTTGATAGCGCATTTATTTTTATTGCt                                                                                      <  1:4813168/100‑1 (MQ=255)   aCTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCtat                                                                                    <  1:124615/100‑1 (MQ=255)            tAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTAcatgc                                                                           <  1:1700780/100‑1 (MQ=255)               ccGGTTCCAAGAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTAcatgcatg                                                                        <  1:1074651/100‑1 (MQ=255)                cGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTAcatgcatgg                                                                       <  1:5596714/100‑2 (MQ=255)                        aaaaTATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAAc                                                               <  1:2904367/100‑1 (MQ=255)                                    acacTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCgg                                                   <  1:2475527/100‑1 (MQ=255)                                    acacTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCgg                                                   <  1:2773952/100‑1 (MQ=255)                                           attatCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAAt                                            >  1:5602217/1‑100 (MQ=255)                                                           aaCACCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTg                            <  1:3143889/100‑1 (MQ=255)                                                              atcTACACCGTTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCt                         <  1:5623088/98‑1 (MQ=255)                                                              acCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCt                         <  1:1852483/100‑1 (MQ=255)                                                              acCTACACCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCt                         <  1:3394470/100‑1 (MQ=255)                                                              acCTACACCATTTTTGATAGCGCATTTATTTTTATTGCGATACTACATGCATGTATATTATCATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCt                         <  1:289289/100‑1 (MQ=255)                                                              acCTACACCACTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCt                         <  1:415641/100‑1 (MQ=255)                                                                  acatCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTagat                     >  1:4478281/1‑100 (MQ=255)                                                                  acacCGTTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGCGGCTagat                     >  1:1181051/1‑100 (MQ=255)                                                                  acacCATTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTagat                     >  1:4227759/1‑100 (MQ=255)                                                                       aTTTTTGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAAc                <  1:2526588/100‑1 (MQ=255)                                                                         ttttGATAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAg              <  1:4230659/100‑1 (MQ=255)                                                                               tAGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTgg        <  1:1177437/100‑1 (MQ=255)                                                                                aGCGCATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGc       <  1:4088399/100‑1 (MQ=255)                                                                                   gcATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGCgga    <  1:2741307/100‑3 (MQ=255)                                                                                   gcATTTATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGCGTg    <  1:2812103/100‑1 (MQ=255)                                                                                    catttattTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGCGTgg   <  1:111723/100‑2 (MQ=255)                                                                                    catttattTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGCGTGa   <  1:3763681/100‑1 (MQ=255)                                                                                     atttatttTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGCGTgcc  <  1:1414149/100‑3 (MQ=255)                                                                                        |                                                                                                 CCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTATTGCTATACTACATGCATGTATATTAACATTAATTATCGGAGGTAATTTTATGAAAGAACGTGGCTATATCGAACAGCTTTGGCGTGAG  >  NC_007793/2499311‑2499494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑