Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	454,603	G→A	G66D (GGT→GAT)	SAUSA300_RS02155 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	454,603	0	G	A	100.0%	19.6 / NA	9	G66D (GGT→GAT)	SAUSA300_RS02155	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/0);  total (9/0)

TACATCAATACTATTCAGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGGTTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACA  >  NC_007793/454543‑454689                                                             |                                                                                       tACATCAATACTATTCAGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACaaaa                                                 >  1:2683020/1‑100 (MQ=255)      cAATACTATTCAGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCaaa                                            >  1:4106006/1‑100 (MQ=255)            gcaggaGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTcc                                      >  1:32019/6‑100 (MQ=255)               tCAGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCACGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAg                                   >  1:1075642/1‑100 (MQ=255)               ccAGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAg                                   >  1:3198024/2‑100 (MQ=255)                cAGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAgt                                  >  1:6588092/1‑100 (MQ=255)                        cgTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTAt                          >  1:2168739/2‑100 (MQ=255)                                             aGTGGCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTAGGAAAAGAtcggaagagca     >  1:3825609/1‑90 (MQ=255)                                                ggCCAAAGTCAAGATTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACa  >  1:531244/1‑100 (MQ=255)                                                             |                                                                                       TACATCAATACTATTCAGGACCTAGTTATGAGTTAACAAATGTTAGTGGCCAAAGTCAAGGTTATTATGACTCTAACGTTTTGCTTTTTAACCAACAAAATCAAAAGTTCCAAGTGTTTTTATTGGGAAAAGATGAAAATAAATACA  >  NC_007793/454543‑454689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑