Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,469,973	T→C	H571R (CAT→CGT)	SAUSA300_RS12695 ←	multidrug MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,469,973	0	T	C	100.0%	111.6 / NA	44	H571R (CAT→CGT)	SAUSA300_RS12695	multidrug MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/38);  total (6/38)

CTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTA  >  NC_007793/2469890‑2470066                                                                                    |                                                                                              cTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTac                                                                               <  1:5564345/100‑1 (MQ=255)        ctttGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGa                                                                        >  1:778808/3‑100 (MQ=255)             cTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGt                                                                   <  1:2595983/100‑1 (MQ=255)                gTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGtttt                                                                <  1:1495409/100‑1 (MQ=255)                      ttaCAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGTTGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTAACTac                                                          <  1:6587677/100‑2 (MQ=255)                                 agtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCaa                                               <  1:4760918/100‑1 (MQ=255)                                  gtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCaaa                                              <  1:4947233/100‑1 (MQ=255)                                  gtaACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCaaa                                              <  1:2463960/100‑1 (MQ=255)                                      cacTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCt                                          <  1:913293/100‑1 (MQ=255)                                       acTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTg                                         <  1:4647537/100‑1 (MQ=255)                                       acTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTg                                         <  1:3671195/100‑1 (MQ=255)                                       acTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTg                                         <  1:327089/100‑1 (MQ=255)                                         tttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTTATTCACGCATATGATCTTGTCCAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGAt                                       <  1:4572637/100‑1 (MQ=255)                                          ttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:3108666/100‑1 (MQ=255)                                          ttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:6448992/100‑1 (MQ=255)                                          ttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:6045269/100‑1 (MQ=255)                                          ttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:2573631/100‑1 (MQ=255)                                          ttCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:546116/100‑1 (MQ=255)                                          cgatctGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATa                                      <  1:2021691/95‑1 (MQ=255)                                                   ccgatctGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGtgttggc                             <  1:6013839/94‑3 (MQ=255)                                                       gatctCCGCTATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGt                         <  1:3067858/96‑1 (MQ=255)                                                         tGTCCGCCATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGtct                       <  1:1505264/100‑3 (MQ=255)                                                             gcccATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTgtgt                   >  1:4526147/3‑100 (MQ=255)                                                             agCCGTATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTttgt                   >  1:2431882/2‑100 (MQ=255)                                                              gCCATGTTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGtgtt                  >  1:1816476/1‑100 (MQ=255)                                                              cccATATTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGtgtt                  >  1:4710733/2‑100 (MQ=255)                                                                 atatTGTGATGCTAATTCACGCATATGATCTTGTACAACTGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTc               <  1:2456134/100‑1 (MQ=255)                                                                 atatTGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTc               <  1:5202185/100‑1 (MQ=255)                                                                   atTGTGATGCTAATGCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCAt             <  1:4954587/100‑1 (MQ=255)                                                                    ttGTGATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCAtt            <  1:919943/100‑1 (MQ=255)                                                                      gtgATGCTAATTCACGGATATGATCTTGTACAACAGGGTTCGTTTTATCTATCTCTTCCCCAAAAGTTGATAAGTGTTGTGTAGTTTGTGTTGTCATTAc          <  1:5364621/100‑1 (MQ=255)                                                                      gtgATGCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTAc          <  1:3306020/100‑1 (MQ=255)                                                                           gCTAATTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTaa     >  1:6392694/1‑100 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTCCAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:6088032/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:1087465/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:4463776/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:5645342/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:3433345/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:3373939/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:6217856/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:1675695/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:1635491/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:802643/100‑1 (MQ=255)                                                                              aaTTCACGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTa  <  1:2880704/100‑1 (MQ=255)                                                                                    |                                                                                              CTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTA  >  NC_007793/2469890‑2470066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑