Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,444,611	T→C	T29T (ACA→ACG)	SAUSA300_RS12565 ←	sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,444,611	0	T	C	100.0%	36.0 / NA	16	T29T (ACA→ACG)	SAUSA300_RS12565	sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/16);  total (0/16)

AGCAAATGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCTGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACT  >  NC_007793/2444524‑2444692                                                                                        |                                                                                  agaaaaTGATTCTTTTCGATTCATTAATAACGCCGCAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCTCCCGTGATAATTCCt                                                                       <  1:1952576/97‑1 (MQ=255)  gCAAATGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTa                                                                      <  1:2063290/100‑1 (MQ=255)       ccgatctcTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCt                                                                 <  1:1941946/96‑1 (MQ=255)          ttCTTTTCGATTCATTAATGACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTatg                                                              <  1:4503944/100‑3 (MQ=255)                      cATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCcaca                                                  <  1:5630118/100‑1 (MQ=255)                       aTTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCcacat                                                 <  1:125241/100‑2 (MQ=255)                       aTTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCcacat                                                 <  1:2527663/100‑2 (MQ=255)                       aTTAATAACGCCACAATGACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACaa                                                 <  1:6542940/100‑1 (MQ=255)                                   acaATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATTCCTACGCCTAAAAACAACGCCACAAATAATAACAAgg                                     <  1:3636583/100‑1 (MQ=255)                                     aaTTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAgggg                                   <  1:2451150/100‑1 (MQ=255)                                      aTTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGa                                  <  1:2316749/100‑1 (MQ=255)                                      aTTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGa                                  <  1:5579869/100‑1 (MQ=255)                                       ttACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGat                                 <  1:5489746/100‑2 (MQ=255)                                       ttACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGaa                                 <  1:4188923/100‑1 (MQ=255)                                         aCCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGaata                               <  1:4200209/100‑1 (MQ=255)                                                                      cATTGAAGTAAAATCACCCGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACt  <  1:3018223/100‑1 (MQ=255)                                                                                        |                                                                                  AGCAAATGATTCTTTTCGATTCATTAATAACGCCACAATTACCGTTATCGTAATTGCAACATTTAATGGCATTGAAGTAAAATCACCTGTGATAATACCTACGCCTAAAAACAACGCCACAAATAATAACAAGGGGAATAATGCCCAAGCATTGCTCTTTTTATGTACT  >  NC_007793/2444524‑2444692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑