Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,443,999	C→A	P233P (CCG→CCT)	SAUSA300_RS12565 ←	sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,443,999	0	C	A	100.0%	40.0 / NA	16	P233P (CCG→CCT)	SAUSA300_RS12565	sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/16);  total (0/16)

TATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTT  >  NC_007793/2443906‑2444086                                                                                              |                                                                                        tATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACa                                                                                   <  1:481851/100‑1 (MQ=255) tATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACa                                                                                   <  1:5127735/100‑1 (MQ=255)  aTGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTAGACCTATTAATGCTAATACCAATACTAAGATATAAggcgggt                                                                                  <  1:3639210/100‑6 (MQ=255)      gaCCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACttt                                                                              <  1:3328226/100‑1 (MQ=255)       aCCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACtttg                                                                             <  1:4728097/100‑2 (MQ=255)          aaTGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAAtt                                                                          <  1:1754961/100‑1 (MQ=255)          aaTGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAAtt                                                                          <  1:5636161/100‑1 (MQ=255)                               tCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGttttag                                                     <  1:21005/100‑3 (MQ=255)                                                                   taatGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATAc                 <  1:3601624/100‑1 (MQ=255)                                                                   taatGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATAc                 <  1:5078063/100‑1 (MQ=255)                                                                   taatGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATAc                 <  1:521550/100‑1 (MQ=255)                                                                   taatGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATAc                 <  1:5406689/100‑1 (MQ=255)                                                                      tGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAgg              <  1:497956/100‑2 (MQ=255)                                                                      tGCTAATACCAATACTAAGATATAAGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGa              <  1:5100837/100‑1 (MQ=255)                                                                       gCTAATACCAATACTAAGATATAAGGTACAACGTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAggc             <  1:4509406/100‑3 (MQ=255)                                                                                  tACTAAGATATAAGGTACAGCTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGtt  <  1:1034581/100‑1 (MQ=255)                                                                                              |                                                                                        TATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTT  >  NC_007793/2443906‑2444086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑