Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,414,505	A→G	L29L (TTA→CTA)	SAUSA300_RS12410 ←	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,414,505	0	A	G	100.0%	43.6 / NA	18	L29L (TTA→CTA)	SAUSA300_RS12410	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/16);  total (2/16)

TCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTGTCATCTTACATCCTCA  >  NC_007793/2414408‑2414601                                                                                                  |                                                                                                 tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                                                                                <  1:3295552/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                                               <  1:1630912/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                                               <  1:5170911/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                                               <  1:3239449/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                                               <  1:5059249/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                                               <  1:4478466/100‑1 (MQ=255)  cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                                                                               <  1:505883/100‑1 (MQ=255)    aTTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCaag                                                                                             <  1:2937812/100‑2 (MQ=255)    aTTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGGTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAat                                                                                             <  1:6483190/100‑1 (MQ=255)                tCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTa                                                                                 <  1:4882647/100‑1 (MQ=255)                tCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTa                                                                                 <  1:5736389/100‑1 (MQ=255)                        cATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGCTCGct                                                                         <  1:2849824/100‑1 (MQ=255)                                  gTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCacac                                                               <  1:5398440/100‑1 (MQ=255)                                     tcttccgatCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCACactac                                                            <  1:5064496/92‑1 (MQ=255)                                                                                 ccgatcTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTGTc                <  1:4525401/95‑1 (MQ=255)                                                                                    acCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTGTCagg             <  1:561010/100‑3 (MQ=255)                                                                                        gTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTGTCATCTTAc         >  1:1201016/1‑100 (MQ=255)                                                                                               gTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTGTCATCTTACATCCTca  >  1:3757872/1‑100 (MQ=255)                                                                                                  |                                                                                                 TCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACACTACTCATTTTCATACTTTTATAAATATGTAGTGAATAATTATCTGTCATCTTACATCCTCA  >  NC_007793/2414408‑2414601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑