Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,414,460	G→C	L44V (CTA→GTA)	SAUSA300_RS12410 ←	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,414,460	0	G	C	100.0%	53.3 / NA	21	L44V (CTA→GTA)	SAUSA300_RS12410	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (4/17);  total (4/17)

TTGCACTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACACTAC  >  NC_007793/2414364‑2414543                                                                                                 |                                                                                    ttGCGCTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATc                                                                                  <  1:2906038/100‑1 (MQ=255) ttGCACTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATc                                                                                  >  1:5399201/1‑100 (MQ=255)         acttCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATAttgtt                                                                          >  1:1038276/1‑100 (MQ=255)                 aacacaacaTACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGGAgatcg                                                                  >  1:555815/1‑98 (MQ=255)                   gacaacaTACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAgattgat                                                                >  1:2848816/2‑100 (MQ=255)                                 tcatcaTTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTg                                                  <  1:3331871/100‑1 (MQ=255)                                          ccATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGtc                                         <  1:6420367/100‑2 (MQ=255)                                             tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                      <  1:3295552/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                     <  1:1630912/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                     <  1:3239449/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                     <  1:4478466/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                     <  1:505883/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                     <  1:5059249/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                     <  1:5170911/100‑1 (MQ=255)                                                aTTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCaag                                   <  1:2937812/100‑2 (MQ=255)                                                aTTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGGTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAat                                   <  1:6483190/100‑1 (MQ=255)                                                            tCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTa                       <  1:5736389/100‑1 (MQ=255)                                                            tCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTa                       <  1:4882647/100‑1 (MQ=255)                                                                    cATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGCTCGct               <  1:2849824/100‑1 (MQ=255)                                                                              gTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCacac     <  1:5398440/100‑1 (MQ=255)                                                                                 tcttccgatCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCACactac  <  1:5064496/92‑1 (MQ=255)                                                                                                 |                                                                                    TTGCACTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACACTAC  >  NC_007793/2414364‑2414543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑