Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,414,448	A→C	Y48D (TAT→GAT)	SAUSA300_RS12410 ←	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,414,448	0	A	C	100.0%	51.7 / NA	20	Y48D (TAT→GAT)	SAUSA300_RS12410	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (4/16);  total (4/16)

TTGCACTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACAC  >  NC_007793/2414364‑2414540                                                                                     |                                                                                             ttGCGCTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATc                                                                               <  1:2906038/100‑1 (MQ=255) ttGCACTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATc                                                                               >  1:5399201/1‑100 (MQ=255)         acttCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATAttgtt                                                                       >  1:1038276/1‑100 (MQ=255)                 aacacaacaTACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGGAgatcg                                                               >  1:555815/1‑98 (MQ=255)                   gacaacaTACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAgattgat                                                             >  1:2848816/2‑100 (MQ=255)                                 tcatcaTTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTg                                               <  1:3331871/100‑1 (MQ=255)                                          ccATCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGtc                                      <  1:6420367/100‑2 (MQ=255)                                             tCAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGcc                                   <  1:3295552/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                  <  1:1630912/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                  <  1:3239449/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                  <  1:4478466/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                  <  1:505883/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                  <  1:5059249/100‑1 (MQ=255)                                              cAATTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCa                                  <  1:5170911/100‑1 (MQ=255)                                                aTTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCaag                                <  1:2937812/100‑2 (MQ=255)                                                aTTTGATACAAATCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGGTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAat                                <  1:6483190/100‑1 (MQ=255)                                                            tCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTa                    <  1:5736389/100‑1 (MQ=255)                                                            tCATTCAACATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTa                    <  1:4882647/100‑1 (MQ=255)                                                                    cATAATAATGGTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGCTCGct            <  1:2849824/100‑1 (MQ=255)                                                                              gTGACATCATTTCTTTTTACATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAGCCAATATATAATTTTTAAGTTCGCTTCCTTCacac  <  1:5398440/100‑1 (MQ=255)                                                                                     |                                                                                             TTGCACTTACTTCAACAACACAACATACACTATCATCATTACCATCAATTTGATACAAATCATTCAACATAATAATGGTGACATAATTTCTTTTTAGATCATATTGTTGTAGATTGATTGAAACTACACCTGTACCTTGTAACCAATATATAATTTTTAAGTTCGCTTCCTTCACAC  >  NC_007793/2414364‑2414540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑