Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,396,927	C→T	E255K (GAA→AAA)	SAUSA300_RS12315 ←	molybdenum ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,396,927	0	C	T	100.0%	46.7 / NA	19	E255K (GAA→AAA)	SAUSA300_RS12315	molybdenum ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/13);  total (6/13)

ACTCGTATTGATATCCAAAAAGGTGTTAAGTCAGGCATGGATTACAACTCCTTATGCTGCAAAGTGGTATTCTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGTATGTGATT  >  NC_007793/2396856‑2397021                                                                        |                                                                                               aCTCGTATTGATATCCAAAAAGGTGTTAAGTCAGGCATGGATTACAACTCCTTATGCTGCAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGAt                                                                    >  1:1551578/1‑100 (MQ=255)             atCCAAAAAGGTGTTAAGTCAGGCATGGATTACAACTCCTTATGCTGCAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATtcc                                                        >  1:1435758/1‑100 (MQ=255)                 aaaaaGGTGTTAAGTCAGGCATGGATTACAACTCCTTATGCTGCAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATtccacat                                                    <  1:5213123/100‑4 (MQ=255)                            aaGTCAGGCATGGATTACAACTCCTTATGCTGCAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCt                                         <  1:3608719/100‑1 (MQ=255)                                     aTGGATTACAACTCCTTATGCTGCAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTa                                <  1:6084809/100‑1 (MQ=255)                                             cgatctcctATGCTGCAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGt                        <  1:5865820/92‑1 (MQ=255)                                             cAACTCCTTATGCTGCAAAGTGGTATTTTTTTAGTATTACTTGAGCTTTATCTGATTTTAAGAATTCCAGCCACTCTTTGGCTAATTTACTATCGGATGt                        <  1:83645/100‑1 (MQ=255)                                              cttccgatctgctgcAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTa                       <  1:1687017/91‑1 (MQ=255)                                                  ccTTATGCTGCAAAGCTTTATTTTTTTCTTATTTCTTTTGCTTTCTCTTCTCTTAAGAATTACTTCCACTCTTTTGCTAATTTACTATCTGATGTAGCAc                   >  1:2828442/1‑100 (MQ=255)                                                           gcAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCga          >  1:2307698/1‑99 (MQ=255)                                                           gcAAAGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGt          <  1:4286109/100‑1 (MQ=255)                                                              aaGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCCCCAGCTTCGTAtg       <  1:4332846/100‑1 (MQ=255)                                                              aaGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGTAtg       <  1:6572281/100‑1 (MQ=255)                                                              aaGTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGTAtg       <  1:113057/100‑1 (MQ=255)                                                                gTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGTAtgtg     <  1:5276744/100‑1 (MQ=255)                                                                gTGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGTAtgtg     <  1:5957920/100‑1 (MQ=255)                                                                atGGTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGTAtcag     >  1:646915/2‑97 (MQ=255)                                                                  ggTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCAACAGCTTCGTATGTGAt   >  1:4349139/1‑100 (MQ=255)                                                                   gTATTTTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCTCCAGCTTCGTATGTGAtg  <  1:4322100/100‑2 (MQ=255)                                                                        |                                                                                               ACTCGTATTGATATCCAAAAAGGTGTTAAGTCAGGCATGGATTACAACTCCTTATGCTGCAAAGTGGTATTCTTTTAGTATTTCTTTAGCTTTATCTGATTTTAAGAATTCCATCCACTCTTTTGCTAATTTACTATCTGATGTAGCACCAGCTTCGTATGTGATT  >  NC_007793/2396856‑2397021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑