Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,388,654	C→T	intergenic (‑87/‑39)	SAUSA300_RS12240 ← / → SAUSA300_RS12245	transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,388,654	0	C	T	100.0%	69.5 / NA	27	intergenic (‑87/‑39)	SAUSA300_RS12240/SAUSA300_RS12245	transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/26);  total (1/26)

TCATAATATGATGCCTCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTA  >  NC_007793/2388564‑2388750                                                                                           |                                                                                                 tCATAATATGATGCCTCCTATGTTTTTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCt                                                                                         <  1:2362911/100‑1 (MQ=255)       cttccgatctcctGTGTTTTTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACa                                                                                   <  1:5976693/92‑1 (MQ=255)                      gTTTTTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTg                                                                    <  1:5643135/100‑1 (MQ=255)                       ttttttCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTgg                                                                   <  1:3321081/100‑1 (MQ=255)                        tttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTggg                                                                  <  1:4166650/97‑2 (MQ=255)                        tttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGc                                                                  <  1:191900/97‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTggat                                                                 <  1:166164/98‑3 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:4599515/98‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:5120948/98‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:400390/98‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:2930627/98‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:2498460/98‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:2070159/98‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:1703429/98‑1 (MQ=255)                         ttttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTGATCTATTACAAATATTAAATTGCTGGcc                                                                 <  1:6238046/98‑2 (MQ=255)                         atttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTCTATCTATTACAAATATTAAATTGCTGGCt                                                                 <  1:15891/98‑1 (MQ=255)                           ttcttAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCtat                                                               <  1:1304964/100‑1 (MQ=255)                                  aTGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGt                                                        <  1:728799/100‑1 (MQ=255)                                    gTTTGTAATTCATATTATTATACAATTCCTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGttt                                                      <  1:2239574/100‑1 (MQ=255)                                    gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGttt                                                      <  1:6180230/100‑1 (MQ=255)                                    gTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGttt                                                      <  1:1198082/100‑1 (MQ=255)                                    gTTTGTAATTCATACTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGttt                                                      <  1:4805800/100‑1 (MQ=255)                                            ttCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTtag                                              <  1:859960/100‑2 (MQ=255)                                                            aTTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACtt                              <  1:4635886/100‑1 (MQ=255)                                                            aTTACTTAAATTTATTATATATCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACtg                              <  1:4342711/100‑2 (MQ=255)                                                                                atCAAACTTAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACTTGTTGTTTATTCGACAACAtt          <  1:4472625/100‑1 (MQ=255)                                                                                        tAGTATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTa  >  1:4883074/1‑100 (MQ=255)                                                                                           |                                                                                                 TCATAATATGATGCCTCCTATGTTTCTTCTTAGATGTTTGTAATTCATATTATTATACAATTACTTAAATTTATTATATATCAAACTTAGCATTTTATCTATTACAAATATTAAATTGCTGGCTATATCTTGTTTAGATTTATATCTTTTGGACAACTTGTTGTTTATTCGACAACATTTATACCTA  >  NC_007793/2388564‑2388750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑