Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,384,995	A→T	intergenic (‑82/‑307)	SAUSA300_RS12215 ← / → SAUSA300_RS12220	lipid II:glycine glycyltransferase/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,384,995	0	A	T	100.0%	210.3 / NA	78	intergenic (‑82/‑307)	SAUSA300_RS12215/SAUSA300_RS12220	lipid II:glycine glycyltransferase/membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/76);  total (2/76)

ATCTTTTCCATGATTTATGATATCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTAAGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATTGTGAATCATTTTATATCTA  >  NC_007793/2384903‑2385089                                                                                             |                                                                                               aTCTTTTCCCTGATTTATGATATCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTTTAatatgtct                                                                                         <  1:4563934/100‑5 (MQ=255)                      cttccgatCTATTTCACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:6486866/93‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATGCAGTGTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAGCTCTTATTTTTGAAATGTcc                                                                    <  1:1779817/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAGCTCTTATTTTTGAAATGTcc                                                                    <  1:2960722/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcg                                                                    <  1:1759153/100‑2 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:5032721/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:573041/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:5852981/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:5945113/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:6244798/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:6129799/100‑1 (MQ=255)                      atCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:6075728/100‑1 (MQ=255)                      atCTCCGTCTATTTAACAATACCTTTAATTATACAGTTCGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcc                                                                    <  1:714426/100‑1 (MQ=255)                       tctcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTcat                                                                   <  1:5929801/100‑3 (MQ=255)                       tctcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCa                                                                   <  1:215310/100‑1 (MQ=255)                       tctcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCa                                                                   <  1:705695/100‑1 (MQ=255)                       tctcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCa                                                                   <  1:3227855/100‑1 (MQ=255)                       tctcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCa                                                                   <  1:3156843/100‑1 (MQ=255)                       tctcC‑TCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCAt                                                                  <  1:1363328/100‑1 (MQ=255)                        ctcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCag                                                                  <  1:1552304/100‑2 (MQ=255)                        ctcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCag                                                                  >  1:4585065/1‑99 (MQ=255)                         ttcTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCaga                                                                 <  1:521329/98‑3 (MQ=255)                         tcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCact                                                                 <  1:5136940/100‑3 (MQ=255)                         tcCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAAAGCTTGTGTATGATTTGAACTCTTA‑TTTTGAAATGTCCAAGc                                                                <  1:193410/100‑1 (MQ=255)                         tcCCTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCAtt                                                                 <  1:2969905/100‑2 (MQ=255)                          ccTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTCTTGAAATGTCCATGc                                                                <  1:3187638/100‑1 (MQ=255)                           ccacTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGca                                                               <  1:430724/97‑2 (MQ=255)                           cTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGca                                                               <  1:5944519/100‑2 (MQ=255)                             tCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTcc                                                             <  1:3514507/100‑1 (MQ=255)                              cTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCa                                                            <  1:3681311/100‑1 (MQ=255)                                 tttAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCagct                                                         <  1:626888/100‑4 (MQ=255)                                       aaTACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTTTTATTTTTGAAATGTCCATGCTCCAATTAATAGt                                                   <  1:510646/100‑1 (MQ=255)                                       aaTACCTTTAATTAGACAGTTTGTATCTTATGGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGt                                                   <  1:4965975/100‑1 (MQ=255)                                         tgccTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGttt                                                 <  1:556112/98‑1 (MQ=255)                                         tACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGttt                                                 <  1:2930487/100‑1 (MQ=255)                                         tACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGttt                                                 <  1:5650151/100‑1 (MQ=255)                                         tACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGttt                                                 <  1:4334096/100‑1 (MQ=255)                                         tACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGttt                                                 <  1:2392857/100‑1 (MQ=255)                                           ccTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAg                                               <  1:6349131/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:5252162/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:899004/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:5785830/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:1394463/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:3080952/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:2737792/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:2663333/100‑1 (MQ=255)                                            cTTTAATTATACAGTTTGTATCTTATAGAGTCGATTCAGAGCTTGTGTATGATTTGAGCTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGc                                              <  1:5878823/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:4750125/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:1046726/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:1174564/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:6649245/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:1378816/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:2015814/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:2083044/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:2594169/100‑1 (MQ=255)                                             tttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:2629933/100‑1 (MQ=255)                                             tttAATTATACAGTGTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:4970330/100‑1 (MQ=255)                                             tttAATTATACAGGTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:6647581/100‑1 (MQ=255)                                             tttAATGATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAAGGTCCATGCTCCAATTAATAGTTTAGCa                                             <  1:5210750/100‑1 (MQ=255)                                              ttAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCaa                                            <  1:5132395/100‑1 (MQ=255)                                                aaTTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGt                                          <  1:5922978/100‑1 (MQ=255)                                                aaTTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCGTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTTCAATTAATAGTTTAGCAAGt                                          <  1:3491591/100‑1 (MQ=255)                                                 aTTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAgag                                         <  1:2957725/100‑3 (MQ=255)                                                 aTTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGtt                                         <  1:6109780/100‑1 (MQ=255)                                                 aTTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGtc                                         <  1:111365/100‑2 (MQ=255)                                                  ttATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTc                                        <  1:5502523/100‑1 (MQ=255)                                                                 cTTATAGTGTCGATCCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCAtttt                         <  1:4939051/100‑1 (MQ=255)                                                                   tataGTGTCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTaa                       >  1:2522324/1‑100 (MQ=255)                                                                        tgtCGATTCAGAGCTTGTGTATGGTTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATtgtg                  <  1:5904195/100‑1 (MQ=255)                                                                        tgtCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATtgtg                  <  1:187810/100‑1 (MQ=255)                                                                        tgtCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATtgtg                  <  1:4234939/100‑1 (MQ=255)                                                                        tgtCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATtgtg                  <  1:5738159/100‑1 (MQ=255)                                                                        tgtCGATTCAGAGATTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGAAAGTTCAAATTTACCCATTTTAATtgtg                  <  1:6079127/100‑1 (MQ=255)                                                                         gtCGATTCAGAGCTTGTGTATGTTTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATtgtgc                 <  1:1621138/100‑2 (MQ=255)                                                                         gtCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAATTCAAATTTACCCATTTTAATTGTGa                 <  1:3900580/100‑1 (MQ=255)                                                                          tCGATTCAGAGCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATTGTGaa                <  1:3638173/100‑1 (MQ=255)                                                                                   agCTTGTGTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATTGTGAATCATTTtat       <  1:2350913/100‑1 (MQ=255)                                                                                       tgtgTATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATTGTGAATCATTTTATATCt   <  1:1426229/100‑1 (MQ=255)                                                                                        gtgtATGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATTGTGAATCATTTTATATCTa  <  1:594023/100‑1 (MQ=255)                                                                                             |                                                                                               ATCTTTTCCATGATTTATGATATCTCCTTCTATTTAACAATACCTTTAATTATACAGTTTGTATCTTATAGTGTCGATTCAGAGCTTGTGTAAGATTTGAACTCTTATTTTTGAAATGTCCATGCTCCAATTAATAGTTTAGCAAGTTCAAATTTACCCATTTTAATTGTGAATCATTTTATATCTA  >  NC_007793/2384903‑2385089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑