Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,381,781	G→A	P584L (CCG→CTG)	SAUSA300_RS12210 ←	multidrug transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,381,781	0	G	A	100.0%	54.1 / NA	20	P584L (CCG→CTG)	SAUSA300_RS12210	multidrug transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/19);  total (1/19)

CAACACTGCTTGCTCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCGGTCCTCCAAATACAATAGTTGCAACCAAAATTAATGTACTTAATATAATTACAATCCACTTATGATTTAATGACCAATGTAATACTTT  >  NC_007793/2381687‑2381869                                                                                               |                                                                                         cAACACTGCTTGCTCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGtcct                                                                                     <  1:825020/100‑1 (MQ=255) cAACACTGCTTGCTCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGtcct                                                                                     <  1:3319338/100‑1 (MQ=255)  aaCACTGCTTGCTCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGtcctg                                                                                    <  1:221829/100‑2 (MQ=255)    cacTGCTTGCTCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGtccctta                                                                                  <  1:5617864/100‑5 (MQ=255)        gcttgctCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCagcat                                                                              <  1:4468632/100‑5 (MQ=255)            gctCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAAt                                                                          <  1:4553924/100‑1 (MQ=255)             ctCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATa                                                                         <  1:2516136/100‑1 (MQ=255)             ccgatcttCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATa                                                                         <  1:2338764/94‑1 (MQ=255)                  tttCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCTCCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATTGTTGc                                                                    <  1:2533899/100‑1 (MQ=255)                  tttCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGc                                                                    <  1:2521501/100‑1 (MQ=255)                  tttCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGc                                                                    <  1:3877015/100‑1 (MQ=255)                  tttCACCAGGCGTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGc                                                                    <  1:3416738/100‑1 (MQ=255)                   ttCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGCa                                                                   <  1:4829957/100‑1 (MQ=255)                   ttCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGCa                                                                   <  1:985430/100‑1 (MQ=255)                   ttCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGACACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGCa                                                                   <  1:4253549/100‑1 (MQ=255)                          ggCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGCAACCaggc                                                            <  1:2799423/100‑4 (MQ=255)                                                           tccgatcttgctgAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGCAACCAAAATTAATGTACTTAATATAATTACAATCCACTTag                           <  1:3109632/92‑2 (MQ=255)                                                               cACCTGCTGAAATAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGCAACCAAAATTAATGTACTTAATATAATTACAATCCACTTATGAtt                       >  1:4636791/1‑100 (MQ=255)                                                                         aaTAAAGCTAGTGCCTAGTCTCAGTCCTCCAAATACAATAGTTGCAACCAAAATTAATGTACTTAATATAATTACAATCCACTTATGATTTAATGACCat             <  1:2501705/100‑2 (MQ=255)                                                                                    cttccgatctcAGTCCTCCAAATACAATAGTTGCAACCAAAATTAATGTACTTAATATAATTACAATCCACTTATGATTTAATGACCAATGTAATACtta  <  1:2459328/93‑2 (MQ=255)                                                                                               |                                                                                         CAACACTGCTTGCTCCGTTTCACCAGGCTTCGGTGTATAAGTAATAGCTAAAAATTTATCGTCACCTGCTGAAATAAAGCTAGTGCCTAGTCTCGGTCCTCCAAATACAATAGTTGCAACCAAAATTAATGTACTTAATATAATTACAATCCACTTATGATTTAATGACCAATGTAATACTTT  >  NC_007793/2381687‑2381869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑