Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,381,180	A→C	T784T (ACT→ACG)	SAUSA300_RS12210 ←	multidrug transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,381,180	0	A	C	100.0%	186.9 / NA	72	T784T (ACT→ACG)	SAUSA300_RS12210	multidrug transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/70);  total (2/70)

TTAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACAGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCATTTTCT  >  NC_007793/2381085‑2381273                                                                                                |                                                                                              ttAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGtga                                                                                           <  1:3594224/100‑3 (MQ=255) ttAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGtag                                                                                           <  1:4370007/100‑3 (MQ=255)  tAAGCGTTCCACCAGTGGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGGTTGCTTATTTTGTTTGACTTTAACATCAACCGtttt                                                                                          <  1:1681128/100‑1 (MQ=255)     tCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTAcc                                                                                       <  1:1983730/100‑1 (MQ=255)        tACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCAtc                                                                                    <  1:6122246/100‑2 (MQ=255)                 tCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAAc                                                                           <  1:3475895/100‑1 (MQ=255)                 tCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAAc                                                                           <  1:6316340/100‑1 (MQ=255)                   ggCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAg                                                                         <  1:5381865/100‑1 (MQ=255)                    gCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAgg                                                                        <  1:4083180/100‑2 (MQ=255)                     cTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAggt                                                                       <  1:1316852/100‑3 (MQ=255)                     cTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTc                                                                       <  1:4052801/100‑1 (MQ=255)                       ttttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGt                                                                     <  1:1177280/100‑1 (MQ=255)                       ttttCAAAGTTATATTATTCAACTTATATTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGt                                                                     <  1:1483024/100‑1 (MQ=255)                         ttCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTaa                                                                   <  1:1627979/100‑1 (MQ=255)                            aaaGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTg                                                                <  1:516033/100‑1 (MQ=255)                                      tattCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTgg                                                      <  1:6580893/100‑1 (MQ=255)                                        ttCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTa                                                    <  1:801446/100‑1 (MQ=255)                                        ttCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTa                                                    <  1:3522116/100‑1 (MQ=255)                                        ttCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTGACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTa                                                    <  1:3065875/100‑1 (MQ=255)                                             cTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGttt                                               <  1:248446/100‑1 (MQ=255)                                                    tctGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTGCCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                        <  1:2731200/100‑1 (MQ=255)                                                    ccgatctgtccTGTTTGCTTATTTTGTTTGACTTTAACATCAGCCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                        <  1:1908775/90‑1 (MQ=255)                                                    ccgatctgatCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTttcattca                                        <  1:1287634/91‑1 (MQ=255)                                                     ctGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCaa                                       >  1:317891/1‑100 (MQ=255)                                                         ttaGTCTGTTTGCTTATTTTGTTTGACGTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTGTTCGGGTAAGTTTTCATTCAAGTGc                                   <  1:2437763/98‑1 (MQ=255)                                                         ccgatCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGc                                   <  1:5802973/96‑1 (MQ=255)                                                         ccAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGc                                   <  1:4363093/100‑1 (MQ=255)                                                         ccAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGc                                   <  1:3638616/100‑1 (MQ=255)                                                         ccAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGc                                   <  1:2240453/100‑1 (MQ=255)                                                         ccAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCCGGTAAGTTTTCATTCAAGTGc                                   <  1:2292912/100‑1 (MQ=255)                                                          ctgTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCa                                  <  1:4697613/98‑1 (MQ=255)                                                          cgatCTGTTTGGTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTgag                                  <  1:2511221/97‑3 (MQ=255)                                                          atctgggTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCa                                  <  1:3039043/94‑1 (MQ=255)                                                           tgTCTGCTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGACGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCac                                 <  1:3218041/99‑2 (MQ=255)                                                           aGTTTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGcct                                 <  1:202596/100‑3 (MQ=255)                                                           aGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAt                                 <  1:2856421/100‑1 (MQ=255)                                                           aGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGGGCAt                                 <  1:1042745/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCACCCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:2844870/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAATCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:6055210/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:5415992/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:6074777/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:6338697/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:1313946/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:141575/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:4165866/100‑1 (MQ=255)                                                            gTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGCTTTCATTCAAGTGCAtt                                <  1:1306271/100‑1 (MQ=255)                                                            gTCTGTTTGCTTAATTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAta                                <  1:6023670/100‑2 (MQ=255)                                                            gTCTGCTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:3937384/100‑1 (MQ=255)                                                            gTCTGCTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAta                                <  1:6007965/100‑2 (MQ=255)                                                            gTCGGTTTGCTTATTTTGTTTGACTTTAACATCATCCGTTTTTCCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:2639437/100‑1 (MQ=255)                                                            gTCCGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                >  1:1622278/1‑100 (MQ=255)                                                            atCTGTTTGTTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:6562470/99‑1 (MQ=255)                                                            atCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:4018017/99‑1 (MQ=255)                                                            atCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:5693915/99‑1 (MQ=255)                                                            atCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTCTTTCTGGTAAGTTTTCATTCAAGTGCAtt                                <  1:3759067/99‑1 (MQ=255)                                                             tCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCTTTTTACCATTTTCTTTAACAGTCGTACCTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTg                               <  1:2688946/100‑1 (MQ=255)                                                             tCTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTg                               <  1:5422166/100‑1 (MQ=255)                                                             tCTGTTTGCTTATTCTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTg                               <  1:4916737/100‑1 (MQ=255)                                                             gcTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCAtta                               <  1:3281315/99‑2 (MQ=255)                                                              ttGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGc                              <  1:3199591/99‑1 (MQ=255)                                                              cTGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGc                              <  1:1004342/100‑1 (MQ=255)                                                               tatttGCTTATTTTGTTTGCCTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:6462143/98‑1 (MQ=255)                                                               tatttGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:484434/98‑1 (MQ=255)                                                               tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:2272383/100‑1 (MQ=255)                                                               tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:5071256/100‑1 (MQ=255)                                                               tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:6108326/100‑1 (MQ=255)                                                               tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:1280261/100‑1 (MQ=255)                                                               tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:2511863/100‑1 (MQ=255)                                                               tGTTTGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCa                             <  1:2317672/100‑1 (MQ=255)                                                                   tGCTTATTTTGTTTGACTTTAACATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAggg                         <  1:5924636/100‑3 (MQ=255)                                                                                       aaCATCAACCGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCAttt     <  1:3477552/100‑1 (MQ=255)                                                                                          aTCAACCGTTTTACCATTTTCCTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTAATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCAtttttg  <  1:1138743/100‑3 (MQ=255)                                                                                                |                                                                                              TTAATCGTACCACCAGTCGGCTTTTTCAAAGTTATATTATTCAACTTATCTTCTGACCAGTCTGTTTGCTTATTTTGTTTGACTTTAACATCAACAGTTTTACCATTTTCTTTAACAGTCGTAACTGTTTTTTCTGGTAAGTTTTCATTCAAGTGCATTGCAAGTTGACTTGCAGAAATACCATTTTCT  >  NC_007793/2381085‑2381273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑