Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,309,270	C→T	G150D (GGT→GAT)	SAUSA300_RS11755 ←	siderophore ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,309,270	0	C	T	100.0%	144.2 / NA	55	G150D (GGT→GAT)	SAUSA300_RS11755	siderophore ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/50);  total (5/50)

TTCAAAATAATAATACCTTCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCACCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGAATGATAATCGTCACA  >  NC_007793/2309174‑2309365                                                                                                 |                                                                                                ttCAAAATAATAATACCTTCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTg                                                                                              <  1:4670290/100‑1 (MQ=255)          ataataCCTTCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGtac                                                                                     <  1:5183524/100‑2 (MQ=255)                  ttCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGtt                                                                             >  1:872679/1‑100 (MQ=255)                   tCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGttt                                                                            <  1:6475724/100‑2 (MQ=255)                   tCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTa                                                                            >  1:1868687/1‑100 (MQ=255)                   tCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTa                                                                            >  1:4289603/1‑100 (MQ=255)                          tactacTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAaccacg                                                                     <  1:34203/100‑2 (MQ=255)                            ctactaAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCta                                                                   <  1:3449449/100‑1 (MQ=255)                               ctaAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATaa                                                                <  1:1819896/100‑1 (MQ=255)                                     aaCAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCAc                                                          <  1:2184417/100‑1 (MQ=255)                                       cAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACTTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCt                                                        <  1:2460472/100‑1 (MQ=255)                                       cAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCt                                                        <  1:1842218/100‑1 (MQ=255)                                        aaaTGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCtt                                                       <  1:1415896/100‑2 (MQ=255)                                        aaaTGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTa                                                       <  1:3285521/100‑1 (MQ=255)                                           tggatgaCCATACCAGCCAATGCTAATTTAATGGGTGTCACACCTTTCGTAGCATCTGAAAGTGTATAAGCAGTTAAACCACCTATAAATGCAACTagag                                                    <  1:4240623/100‑4 (MQ=255)                                            ggatggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATcac                                                   <  1:2302054/100‑1 (MQ=255)                                            ggatggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATcac                                                   <  1:2519363/100‑1 (MQ=255)                                            ggatggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATcac                                                   <  1:35048/100‑1 (MQ=255)                                            ggatggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATcac                                                   <  1:1706275/100‑1 (MQ=255)                                              atggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATcacag                                                 <  1:2054302/100‑2 (MQ=255)                                              atggCCATACCAGCCAATGCTAATTTAATGGGTGTCACACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCacac                                                 <  1:3184031/100‑1 (MQ=255)                                               tggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATcaccac                                                <  1:3914315/100‑4 (MQ=255)                                                ggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCt                                               <  1:3826050/100‑1 (MQ=255)                                                ggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCt                                               <  1:3159719/100‑1 (MQ=255)                                                ggCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCt                                               <  1:1405762/100‑1 (MQ=255)                                                 gCCATCCCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTa                                              <  1:6562966/100‑1 (MQ=255)                                                 gCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTa                                              <  1:3056507/100‑1 (MQ=255)                                                 gCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTa                                              <  1:5945094/100‑1 (MQ=255)                                                 gCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTa                                              <  1:4536865/100‑1 (MQ=255)                                                 gCCACACCAGCCAATGCTAATTTACTGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACCCCTa                                              <  1:844201/100‑1 (MQ=255)                                                  ccATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTag                                             <  1:2368075/100‑2 (MQ=255)                                                   cATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTaga                                            <  1:588311/100‑3 (MQ=255)                                                    aTACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAat                                           <  1:5999198/100‑1 (MQ=255)                                                      aCCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAatat                                         >  1:2229241/1‑100 (MQ=255)                                                       ccAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAatagg                                        <  1:5941877/100‑3 (MQ=255)                                                           ctaaTGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGc                                    >  1:933683/3‑100 (MQ=255)                                                           ccAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGc                                    <  1:949589/100‑1 (MQ=255)                                                             aaTGATAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCat                                  <  1:5311970/100‑1 (MQ=255)                                                              aTGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCata                                 <  1:976047/100‑1 (MQ=255)                                                              aTGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCata                                 <  1:6586772/100‑1 (MQ=255)                                                              aTGCTAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCata                                 <  1:1488737/100‑1 (MQ=255)                                                                  tAATTTAATGGGTGTCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAATCCACCTATAAATGCACCTATCACACCTAAATATAATGCATAatat                             <  1:4464290/100‑1 (MQ=255)                                                                             gtgtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCCCACCTAAATATAATGCATAATATTCTAATGCTgg                  <  1:6258960/100‑1 (MQ=255)                                                                             gtgtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACTCCTAAATATAATGCATAATATTCTAATGCTgg                  <  1:4403488/100‑1 (MQ=255)                                                                             gtgtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATGATATTCTAATGCTgg                  <  1:3667083/100‑1 (MQ=255)                                                                             gtgtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTgg                  <  1:6031307/100‑1 (MQ=255)                                                                             gtgtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTgg                  <  1:2364947/100‑1 (MQ=255)                                                                              tgtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGa                 <  1:5754826/100‑1 (MQ=255)                                                                              tgtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGa                 <  1:206892/100‑1 (MQ=255)                                                                              tgtCATACCTTTCGGAGCATCTGAAAGTGTATAAACAGTTAAACCACCTAGAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTggg                 <  1:4788378/100‑2 (MQ=255)                                                                               gtCATACCTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCGCACCTAAATATAATGCATAATATTCTAATGCTGGaa                <  1:3519159/100‑1 (MQ=255)                                                                                      cTTTCGTAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGAATGaggcc         <  1:1557657/100‑5 (MQ=255)                                                                                            tAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGAATGATAATCGTcac   <  1:517153/100‑1 (MQ=255)                                                                                            tAGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGAATGATAATCGTcac   <  1:2813099/100‑1 (MQ=255)                                                                                             aGCATCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGAATGATAATCGTcaca  <  1:2659298/100‑1 (MQ=255)                                                                                                 |                                                                                                TTCAAAATAATAATACCTTCGGTCATACTACTAAAAAACAAATGGATGGCCATACCAGCCAATGCTAATTTAATGGGTGTCATACCTTTCGTAGCACCTGAAAGTGTATAAACAGTTAAACCACCTATAAATGCACCTATCACACCTAAATATAATGCATAATATTCTAATGCTGGAATGATAATCGTCACA  >  NC_007793/2309174‑2309365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑