Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,300,219	G→A	A156V (GCT→GTT)	SAUSA300_RS11710 ←	multidrug MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,300,219	0	G	A	100.0%	58.4 / NA	23	A156V (GCT→GTT)	SAUSA300_RS11710	multidrug MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/21);  total (2/21)

TTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTAAAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAGCTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCTAGTCCCATTGCAAAACCTCGATGTTCTTTACTGAACAA  >  NC_007793/2300124‑2300301                                                                                                |                                                                                   ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                                                                                <  1:5483415/100‑1 (MQ=255) ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                                                                                <  1:2146241/100‑1 (MQ=255) ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                                                                                <  1:4517380/100‑1 (MQ=255) ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                                                                                <  1:3470329/100‑1 (MQ=255)      accaaaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTc                                                                           <  1:1184360/100‑1 (MQ=255)          aaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTAt                                                                       <  1:5032218/100‑1 (MQ=255)                 aaCAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTggg                                                                <  1:3569001/100‑2 (MQ=255)                  aCAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTg                                                               <  1:6085857/100‑1 (MQ=255)                  aCAAAGGCAAGTATAGCAATTCCTACAATTATAATAAAGGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACGGTAGGTCCTATTGCTGGTg                                                               <  1:4095052/100‑1 (MQ=255)                   cAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGc                                                              <  1:5547966/100‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:3241618/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:3922062/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:6468397/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:1173779/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:4314311/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:3202775/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:1962120/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:510267/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:5162589/97‑1 (MQ=255)                                         tacaaTTATAATAAATGGAACTCGCCAACTCACTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCt                                        <  1:716123/97‑1 (MQ=255)                                            aaTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCGATTGCTGGTGCAAATTGAATTACTAAACCTGCTAGt                                     <  1:5011129/100‑1 (MQ=255)                                             cttATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCGAgat                                    >  1:1768851/2‑98 (MQ=255)                                             aTTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCTAggt                                    <  1:31580/100‑3 (MQ=255)                                                    taaATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCTAGTccccaaag                             <  1:6205978/100‑6 (MQ=255)                                                    taaATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCTAGTCCCATTGc                             <  1:5157996/100‑1 (MQ=255)                                                    taaATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCTAGTCCCATTGc                             <  1:3970209/100‑1 (MQ=255)                                                                               cAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCTAGTCCCATTGCAAAACCTCGATGTTCTTTACTGAACaa  >  1:3820380/1‑100 (MQ=255)                                                                                                |                                                                                   TTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTAAAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAGCTGTAGGTCCTATTGCTGGTGCAAATTGAATTACTAAACCTGCTAGTCCCATTGCAAAACCTCGATGTTCTTTACTGAACAA  >  NC_007793/2300124‑2300301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑