Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,300,166	A→C	L174V (TTA→GTA)	SAUSA300_RS11710 ←	multidrug MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,300,166	0	A	C	100.0%	100.7 / NA	37	L174V (TTA→GTA)	SAUSA300_RS11710	multidrug MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/37);  total (0/37)

ACATTACTGAACGCTTATCTAATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTAAAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAGCTGTAGGTCCTATTGCTGGTGC  >  NC_007793/2300070‑2300241                                                                                                 |                                                                            aCATTACTGAACGCTTATCTAATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTacaaa                                                                          <  1:646957/100‑2 (MQ=255) aCATTACTGAACGCCTATCTAATTTCGTATATTTCACTTCGTTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAAt                                                                          <  1:4803461/100‑1 (MQ=255)            cGCTTATCTAATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATaaagg                                                               <  1:2211104/100‑3 (MQ=255)             ttccgatctttttCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATgg                                                              <  1:936708/90‑1 (MQ=255)             gCTTATCTAATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATgg                                                              <  1:5241237/100‑1 (MQ=255)               ttATCTAATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGaa                                                            <  1:172919/100‑1 (MQ=255)                     aaTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGcc                                                      <  1:4495897/100‑1 (MQ=255)                     aaTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGcc                                                      <  1:5658227/100‑1 (MQ=255)                     aaTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGcc                                                      <  1:3821283/100‑1 (MQ=255)                     aaTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGcc                                                      <  1:3552329/100‑1 (MQ=255)                      aTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCa                                                     <  1:5157302/100‑1 (MQ=255)                      aTTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCa                                                     <  1:606415/100‑1 (MQ=255)                        tacGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCagt                                                   <  1:6590141/98‑3 (MQ=255)                         tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCatag                                                  <  1:2802693/100‑4 (MQ=255)                         tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCacaa                                                  <  1:5186513/100‑4 (MQ=255)                         tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAGCt                                                  <  1:1410197/100‑1 (MQ=255)                         tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACt                                                  <  1:4277836/100‑1 (MQ=255)                         tCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACt                                                  <  1:5068554/100‑1 (MQ=255)                             atatTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGcc                                              <  1:5544596/100‑2 (MQ=255)                             atatTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCt                                              <  1:271403/100‑1 (MQ=255)                             atatTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCt                                              <  1:2353788/100‑1 (MQ=255)                               atTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGTTTg                                            <  1:6062853/100‑1 (MQ=255)                               atTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTg                                            <  1:6185852/100‑1 (MQ=255)                               atTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTg                                            <  1:1025363/100‑1 (MQ=255)                                  tCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATc                                         <  1:2065016/100‑1 (MQ=255)                                                 tCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgt                          <  1:5386958/100‑1 (MQ=255)                                                  cGAGATTGAACCCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACCATTATAATAAATGGAACTCGCCAACCCGCTTGATCAATAATTAATCctgta                         <  1:1979084/100‑1 (MQ=255)                                                  cGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgta                         <  1:5950415/100‑1 (MQ=255)                                                       ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                    <  1:5483415/100‑1 (MQ=255)                                                       ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                    <  1:4517380/100‑1 (MQ=255)                                                       ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                    <  1:3470329/100‑1 (MQ=255)                                                       ttGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCctgtaactgt                    <  1:2146241/100‑1 (MQ=255)                                                            accaaaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTc               <  1:1184360/100‑1 (MQ=255)                                                                aaccGAAAACAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTAt           <  1:5032218/100‑1 (MQ=255)                                                                       aaCAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTggg    <  1:3569001/100‑2 (MQ=255)                                                                        aCAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTg   <  1:6085857/100‑1 (MQ=255)                                                                        aCAAAGGCAAGTATAGCAATTCCTACAATTATAATAAAGGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACGGTAGGTCCTATTGCTGGTg   <  1:4095052/100‑1 (MQ=255)                                                                         cAAAGGCAAGTATAGCAATTCCTACAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAACTGTAGGTCCTATTGCTGGTGc  <  1:5547966/100‑1 (MQ=255)                                                                                                 |                                                                            ACATTACTGAACGCTTATCTAATTTCGTATATTTCACTTCATTGTAACTCGAGATTGAAACCAAACCGAAAACAAAGGCAAGTATAGCAATTCCTAAAATTATAATAAATGGAACTCGCCAACTCGCTTGATCAATAATTAATCCTGTAGCTGTAGGTCCTATTGCTGGTGC  >  NC_007793/2300070‑2300241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑