Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,276,564	A→G	E56G (GAA→GGA)	SAUSA300_RS11615 →	PTS mannitol transporter subunit IIA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,276,564	0	A	G	100.0%	10.0 / NA	6	E56G (GAA→GGA)	SAUSA300_RS11615	PTS mannitol transporter subunit IIA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/0);  total (6/0)

AAGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGAAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGACT  >  NC_007793/2276480‑2276659                                                                                     |                                                                                                aaGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAAt                                                                                  >  1:2444919/1‑100 (MQ=255)                        gTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGAtcggaaga                                                           >  1:3612175/1‑93 (MQ=255)                                                                       aTCAACATTTATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGaa            >  1:3990972/1‑100 (MQ=255)                                                                               ttATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGa    >  1:3380952/1‑100 (MQ=255)                                                                                tATGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGAc   >  1:3924963/1‑100 (MQ=255)                                                                                 aTGGGAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGACt  >  1:2300599/1‑100 (MQ=255)                                                                                     |                                                                                                AAGCCTTAGTTGATAGTGGTGCTGTAACAGATGCTTATATTCAAGCAATGAAAGATCGTGAGCAAGTCGTATCAACATTTATGGAAAATGGCTTAGCAATTCCTCATGGCACAGATGAAGCTAAAACAAATGTGATTCACTCAGGTTTAACATTATTACAAATCCCTGAAGGCGTTGACT  >  NC_007793/2276480‑2276659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑