Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,240,223 T→C A247A (GCA→GCG)  SAUSA300_RS11440 ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,240,2230TC96.2% 127.3 / ‑2.9 53A247A (GCA→GCG) SAUSA300_RS11440UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (2/49);  total (2/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TCTCTTACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACATGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCATTGATTTTAATT  >  NC_007793/2240125‑2240319
                                                                                                  |                                                                                                
tctcTT‑CATCAACATTCACACCTAGTTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAtgc                                                                                                <  1:5798978/100‑1 (MQ=255)
   ctTACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgcag                                                                                              <  1:4413834/100‑3 (MQ=255)
    tttcATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:1720727/97‑1 (MQ=255)
    ttgcgtCAACATTCACTCCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:5596812/95‑1 (MQ=255)
    ttgcATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:1905872/97‑1 (MQ=255)
    ttACGTCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:5492748/100‑1 (MQ=255)
    ttACGTCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATGTTCTCCACAcgctgc                                                                                             <  1:5256345/100‑1 (MQ=255)
    ttACGTCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACACTTTCTCCACAcgctgc                                                                                             <  1:5250432/100‑1 (MQ=255)
    ttACCTCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:5533630/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCTCTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:3536138/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACGTGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:1223088/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:5306371/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:543940/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:2067211/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:2091511/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:5024526/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:618532/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:4396263/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:4360378/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:2321645/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:4046155/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:3873045/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:3015924/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:2536390/100‑1 (MQ=255)
    ttACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACAAGTTTCGGAACAATATTAGTTAATATAACAATTTCTCCACAcgctgc                                                                                             <  1:939308/100‑1 (MQ=255)
    tccgatcttcATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgc                                                                                             <  1:6119280/91‑1 (MQ=255)
     tACGTCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgct                                                                                            <  1:2246457/100‑2 (MQ=255)
     tACATCAACATTCACTCCTAATTCGCTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTAGTTAATATGACATTTTCTCCACAcgcagct                                                                                            <  1:513544/100‑2 (MQ=255)
     tACACCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCa                                                                                            >  1:2361459/1‑100 (MQ=255)
     ccgatctgcATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCa                                                                                            <  1:3320510/92‑1 (MQ=255)
      ccATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgcttgca                                                                                           <  1:3070581/99‑5 (MQ=255)
      atgtCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAg                                                                                           <  1:5169948/97‑1 (MQ=255)
      acgtCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAg                                                                                           <  1:2354204/97‑1 (MQ=255)
      acgtCAACATTCACACCTAAGTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAg                                                                                           <  1:5925455/97‑1 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACAcgctgctg                                                                                           <  1:5788817/100‑3 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCat                                                                                           <  1:5373865/100‑2 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCat                                                                                           <  1:2719254/100‑2 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCac                                                                                           <  1:1273989/100‑2 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCaa                                                                                           <  1:3264138/100‑2 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAg                                                                                           <  1:245230/100‑1 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAg                                                                                           <  1:5320665/100‑1 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAg                                                                                           <  1:4319694/100‑1 (MQ=255)
      aCATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAg                                                                                           <  1:4816152/100‑1 (MQ=255)
       gatctgcATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAgg                                                                                          <  1:3521932/94‑2 (MQ=255)
       cagcAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACAGGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGc                                                                                          <  1:3406081/97‑1 (MQ=255)
       atctaaCATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGc                                                                                          <  1:2595760/96‑1 (MQ=255)
        ctCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCg                                                                                         <  1:5350074/99‑1 (MQ=255)
        aTCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCg                                                                                         <  1:4609574/100‑1 (MQ=255)
         ccgatctccaccCCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGa                                                                                        <  1:2456077/92‑1 (MQ=255)
                tCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACat                                                                                 <  1:531999/100‑1 (MQ=255)
                      cTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATAtgt                                                                           <  1:1924862/100‑1 (MQ=255)
                       tAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATAtgtg                                                                          <  1:1554557/100‑1 (MQ=255)
                          ttCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCt                                                                       <  1:6524257/100‑1 (MQ=255)
                          ttCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCt                                                                       <  1:1489109/100‑1 (MQ=255)
                          ttCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCt                                                                       <  1:2265165/100‑1 (MQ=255)
                          ttCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCt                                                                       <  1:420761/100‑1 (MQ=255)
                            cACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCtca                                                                     <  1:6091031/100‑3 (MQ=255)
                            cACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCtag                                                                     <  1:3301940/100‑3 (MQ=255)
                                     tAGCAGTTAATGTTTCTACTTGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCt                                                            <  1:4679289/100‑1 (MQ=255)
                                     tAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCt                                                            <  1:2595774/100‑1 (MQ=255)
                                        cAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACCCGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATc                                                         <  1:1983991/100‑1 (MQ=255)
                                        cAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTagg                                                         <  1:2256761/100‑3 (MQ=255)
                                        cAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATc                                                         <  1:1575561/100‑1 (MQ=255)
                                        cAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATc                                                         <  1:2994697/100‑1 (MQ=255)
                                        cAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATc                                                         <  1:5765535/100‑1 (MQ=255)
                                            tAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCGGGt                                                     <  1:5560855/100‑1 (MQ=255)
                                              aTGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGtat                                                   <  1:2348869/100‑1 (MQ=255)
                                                     tACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGa                                            >  1:4379908/1‑100 (MQ=255)
                                                                gAACAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAAcc                                 <  1:2106007/100‑1 (MQ=255)
                                                                  aCAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCaa                               <  1:2535200/100‑2 (MQ=255)
                                                                  aCAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCAt                               <  1:4673381/100‑1 (MQ=255)
                                                                  aCAATATTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCAt                               <  1:3800788/100‑1 (MQ=255)
                                                                     atatTATTTAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTa                            <  1:4244585/100‑1 (MQ=255)
                                                                           tttAATATAACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCtt                      <  1:1043709/100‑1 (MQ=255)
                                                                                tataACATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTgcac                 <  1:5685166/100‑4 (MQ=255)
                                                                                   aaCATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTAATACTCAGAACCATGTAATTCTTTGACGCCa              <  1:172375/100‑1 (MQ=255)
                                                                                    aCATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCAt             <  1:1789833/100‑1 (MQ=255)
                                                                                     tccgatctcCACATGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCAtc            <  1:5091538/95‑2 (MQ=255)
                                                                                     cATTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCAtt            <  1:2496642/100‑1 (MQ=255)
                                                                                      ccgatctcCACATGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCAtag           <  1:3593756/96‑3 (MQ=255)
                                                                                      ccgatctcCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCACGTAATTCTTTGACGCCATTg           <  1:1623697/96‑1 (MQ=255)
                                                                                      atctcctcCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCATTg           <  1:5993430/95‑1 (MQ=255)
                                                                                      aTTTTCTCCACACGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGTTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCATTg           <  1:4382950/100‑1 (MQ=255)
                                                                                       tccgatctacacGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCATTGa          <  1:331564/92‑1 (MQ=255)
                                                                                               acacGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCATTGATTTTAAtt  >  1:5120957/1‑100 (MQ=255)
                                                                                                  |                                                                                                
TCTCTTACATCAACATTCACACCTAATTCACTAAATTTAGCAGTTAATGTTTCTACATGTTTCGGAACAATATTATTTAATATAACATTTTCTCCACATGCTGCAGCGATACACATATATGTGCCTGCTTCAATTCTATCAGGTATAACTTGATACTCAGAACCATGTAATTCTTTGACGCCATTGATTTTAATT  >  NC_007793/2240125‑2240319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑