Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	24,199	A→T	noncoding (22/75 nt)	SAUSA300_RS00095 →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	24,199	0	A	T	88.9%	13.4 / ‑3.6	9	noncoding (22/75 nt)	SAUSA300_RS00095	tRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (7/1);  total (8/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGAACGGCTCCTTGGTCAAGCGGTTAAGACACCGCCCTTTCACGGCGGTAACACGGGTTCGAGTCCCGTAGGAGTCACCATTTTTTAGGTCTCGTAGTGTAGCGGTTAACACG  >  NC_007793/24171‑24285                             |                                                                                       tAAGAACGGCTCCTTGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACACGGGTTTGAGTCCCGTAGGCGTCACCATTTTTTTGGTCTCGTTgt                 >  1:5395813/1‑100 (MQ=17)   tcttcGGCTCCTTGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACACGGGTTCGAGTCCCGTAGGAGTCACCATTTTTTAGGTCTCGCAgtgt               <  1:6223579/96‑1 (MQ=255)      caggCTCCTTGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACACGGGTTTGTGTCCCGTAAGAGACACCCTTTTTTAGGTCCCGCAGTGGAGc            >  1:5761099/3‑100 (MQ=14)        ggCTCCTTGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACACGGGTTCGAGTCCCGTAGGAGTCACCATTTTTTAGGTCTCGTAGTGTAGCgg          >  1:3963821/1‑100 (MQ=255)        ggCTCCTTGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACACGGGTTCGAGTCCCGTAGGAGTCACCATTTTTTAGGCCTCGTAgatcgaaga          >  1:5809800/1‑92 (MQ=17)        ggCTCCTTGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACAAGGGTTCGCGTGCCGTAGGAGTCCCCCTTTTTTAGGACTCGTAgatcggaag          >  1:5879108/1‑92 (MQ=14)            ccTTGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACACGGGTTCGAGTCCCGGAGGGGTCACCATTTTTTAGGTCTCGTTGTGTAGCGGTTaa      >  1:1213710/1‑100 (MQ=18)               tGGTCAAGCGGTTATGACACCGCCCTTTCACGGCGGTAACACGGGTTCGAGTCCCGTAGGAGTCACCATTTTTTAGGGCTCGTAGTGTAGCGGTTAacac   >  1:477189/1‑100 (MQ=255)                ggTCAAGCGGTTAAGACACCGCCCTTTCACGGCGGTAAAAAGGGATCCAGACCCGCAGGGGTCAACTTTTTTTATGGCTCGTAGTGTTGCGGGTaaaaag  >  1:5182476/1‑96 (MQ=9)                             |                                                                                       TAAGAACGGCTCCTTGGTCAAGCGGTTAAGACACCGCCCTTTCACGGCGGTAACACGGGTTCGAGTCCCGTAGGAGTCACCATTTTTTAGGTCTCGTAGTGTAGCGGTTAACACG  >  NC_007793/24171‑24285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑