Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,196,542	C→T	A237A (GCG→GCA)	SAUSA300_RS11190 ←	potassium‑transporting ATPase A chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,196,542	0	C	T	100.0%	11.2 / NA	6	A237A (GCG→GCA)	SAUSA300_RS11190	potassium‑transporting ATPase A chain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/4);  total (2/4)

TAACATGCGACCAAATAAGAACAACATTGACATAGGAAGTAACATCATACTGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTCGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCAAGATGTTTTATTGATTCAA  >  NC_007793/2196443‑2196600                                                                                                    |                                                           tAACATGCGACCAAATAAGAACAACATTGACATAGGAAGTAACATCATACTGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTc                                                            <  1:2237926/100‑1 (MQ=255)                                        tAACATCATACTGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTTGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCa                     <  1:3069191/100‑1 (MQ=255)                                        tAACATCATACTGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTTGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCa                     <  1:3285822/100‑1 (MQ=255)                                        tAACATCATACTGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTTGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCa                     <  1:5081404/100‑1 (MQ=255)                                                 aCTGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTTGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCAAGATGtttt            >  1:1159440/1‑100 (MQ=255)                                                   tGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTTGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCAAGATGTTTTAt          >  1:2543396/1‑100 (MQ=255)                                                           tCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTTGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCAAGATTTTTTATTGATTCag  <  1:5643460/100‑2 (MQ=255)                                                                                                    |                                                           TAACATGCGACCAAATAAGAACAACATTGACATAGGAAGTAACATCATACTGCCCATTTCTATAAAATTGCTCCAAATATTTGGATTTTCAAAAGGTGTCGCAGAATTTCCTGCTAAAAATCCTCCACCATTCGTACCAAGATGTTTTATTGATTCAA  >  NC_007793/2196443‑2196600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑