Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,188,603	G→A	intergenic (‑56/+29)	SAUSA300_RS11150 ← / ← SAUSA300_RS11155	antitoxin MazE/alanine racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,188,603	0	G	A	100.0%	218.5 / NA	76	intergenic (‑56/+29)	SAUSA300_RS11150/SAUSA300_RS11155	antitoxin MazE/alanine racemase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/67);  total (9/67)

AAGCTATGACTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATGATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCAA  >  NC_007793/2188513‑2188691                                                                                           |                                                                                         aaGCTATGACTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAAt                                                                                 >  1:5045137/1‑100 (MQ=255) aaGCTATGACTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAAt                                                                                 >  1:4320641/1‑100 (MQ=255)     tATGACTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAAttttt                                                                             <  1:5150174/100‑2 (MQ=255)      aTGACTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCGTTTGATTCCAAAAGTTATTAAATGTCCTTTAATTACTCTAATAATAACAAATTTtat                                                                            <  1:6562980/100‑1 (MQ=255)      aTGACTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTtat                                                                            <  1:5217988/100‑1 (MQ=255)          cTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGc                                                                        <  1:1846179/100‑1 (MQ=255)           ttCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAACTTTTATATGCa                                                                       <  1:3607652/100‑1 (MQ=255)            tCTGTTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAc                                                                      >  1:4142417/1‑100 (MQ=255)            tCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAc                                                                      <  1:6148882/100‑1 (MQ=255)            tCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAc                                                                      <  1:6191159/100‑1 (MQ=255)            tCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAc                                                                      <  1:6620966/100‑1 (MQ=255)            tCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAc                                                                      <  1:551540/100‑1 (MQ=255)            tCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAc                                                                      <  1:1668077/100‑1 (MQ=255)            tCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAACTTTATATGCAc                                                                      <  1:4273351/100‑1 (MQ=255)             cTATTTTGACTAAGAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACt                                                                     <  1:5004277/100‑1 (MQ=255)             cTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAATAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCata                                                                     <  1:1646119/100‑3 (MQ=255)             cTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAcg                                                                     <  1:4883014/100‑2 (MQ=255)             cTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCAca                                                                     <  1:4253435/100‑2 (MQ=255)              tagtttGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTg                                                                    <  1:5007015/97‑1 (MQ=255)              tATTTTGACTAAAAGATAACATATGAGAGCCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTg                                                                    <  1:1105671/100‑1 (MQ=255)              tATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTg                                                                    <  1:1848686/100‑1 (MQ=255)              tATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTg                                                                    <  1:4489180/100‑1 (MQ=255)              tATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTg                                                                    <  1:1241696/100‑1 (MQ=255)              tATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCGTTAATTACTCTAATAATAACAAATTTTATATGCACTg                                                                    <  1:3760426/100‑1 (MQ=255)              gatcttGGCTAAAAGATAACATATGAGAACCTCCAGTTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTg                                                                    <  1:3538141/96‑1 (MQ=255)               aTTTTGACTAAAAGATAACATATGAGATCCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGa                                                                   <  1:14389/100‑1 (MQ=255)               aTTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTgg                                                                   <  1:5884165/100‑2 (MQ=255)               aTTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGa                                                                   <  1:2312029/100‑1 (MQ=255)                 tttGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGAcc                                                                 <  1:3657599/100‑1 (MQ=255)                 tttGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGAcc                                                                 <  1:5065491/100‑1 (MQ=255)                  ctGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTTTAATAATAACAAATTTTATATGCACTGACCa                                                                >  1:3744520/2‑100 (MQ=255)                   tGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCat                                                               <  1:3508282/100‑1 (MQ=255)                   tGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCat                                                               <  1:2231017/100‑1 (MQ=255)                   tGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCat                                                               <  1:3925599/100‑1 (MQ=255)                   tGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCat                                                               <  1:411965/100‑1 (MQ=255)                    gACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCatg                                                              <  1:5603913/100‑2 (MQ=255)                    gACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCatg                                                              <  1:5708700/100‑2 (MQ=255)                    gACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCatg                                                              <  1:1841735/100‑2 (MQ=255)                    gACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCata                                                              <  1:2136271/100‑1 (MQ=255)                     aCTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCatat                                                             >  1:5375728/1‑100 (MQ=255)                     aCTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCagcg                                                             <  1:1995181/100‑4 (MQ=255)                      cTAAAAGATAACATATGAGACCCTCTAATTGATTCCAAAAGTTATTAAATGACCTTTAATTTCTCTAATATTCACAAATTTTATATGCACTGCCCataat                                                            <  1:4576618/100‑3 (MQ=255)                      cTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCAtatt                                                            <  1:1602043/100‑1 (MQ=255)                       tAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCatatgt                                                           <  1:20776/100‑3 (MQ=255)                       tAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCataggg                                                           <  1:1684444/100‑4 (MQ=255)                       tAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCAtatta                                                           <  1:6387387/100‑1 (MQ=255)                        aaaaGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCAtattat                                                          >  1:5841789/1‑100 (MQ=255)                                           ccTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTa                                       >  1:6262574/1‑100 (MQ=255)                                              ccAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACaa                                    >  1:1145960/1‑100 (MQ=255)                                              ccAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACaa                                    >  1:2238163/1‑100 (MQ=255)                                                     aTTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCGCTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTg                             <  1:602521/100‑1 (MQ=255)                                                     aTTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTg                             <  1:1404410/100‑1 (MQ=255)                                                           aaaGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCAcc                       <  1:3616934/100‑1 (MQ=255)                                                            aaGTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACca                      <  1:4117831/100‑1 (MQ=255)                                                              gTTATTAAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACcatc                    <  1:4490016/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTGTATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:6283174/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCCCCATCATGAt               <  1:2212654/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:3260820/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:2206172/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:6128048/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:2278012/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:2151283/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:5427120/100‑1 (MQ=255)                                                                   taAATGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:14018/100‑1 (MQ=255)                                                                   gatctGACCTTTAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:3425271/96‑1 (MQ=255)                                                                   cgatctgccTTTAATTACTCTAATAATAACAAAGTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:4509866/93‑1 (MQ=255)                                                                   atcttGACCTTTAATTACTCTAATAATAACAAATTTTATATGCGCTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGAt               <  1:3736212/96‑1 (MQ=255)                                                                             ttAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCgg     <  1:2462119/100‑1 (MQ=255)                                                                              tAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGc    <  1:3252316/100‑1 (MQ=255)                                                                              tAATTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGc    <  1:5365780/100‑1 (MQ=255)                                                                               aaTTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCa   <  1:5741881/100‑1 (MQ=255)                                                                               aaTTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCa   <  1:5951568/100‑1 (MQ=255)                                                                                cttgctctAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCat  <  1:4428951/96‑2 (MQ=255)                                                                                aTTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCaa  <  1:4904641/100‑1 (MQ=255)                                                                                aTTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCaa  <  1:3752617/100‑1 (MQ=255)                                                                                aTTACTCTAATAATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCaa  <  1:6626876/100‑1 (MQ=255)                                                                                           |                                                                                         AAGCTATGACTTCTATTTTGACTAAAAGATAACATATGAGAACCTCCAATTGATTCCAAAAGTTATTAAATGACCTTTAATTACTCTAATGATAACAAATTTTATATGCACTGACCATATTATTTTAACAATTCATTTGTTACAAAACGTTGATCACCATCATGATAGATTCGCGGCAA  >  NC_007793/2188513‑2188691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑