Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,158,122	T→G	C107W (TGT→TGG)	SAUSA300_RS10995 →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,158,122	0	T	G	100.0%	27.9 / NA	12	C107W (TGT→TGG)	SAUSA300_RS10995	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/1);  total (11/1)

CTAATGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGTTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAATTAGAAGGA  >  NC_007793/2158037‑2158218                                                                                      |                                                                                                 cTAATGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTGAATCAAAGAAGAAACAGATTGGTTATCAAAACATga                                                                                    <  1:3009012/100‑2 (MQ=255)       ataCAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGa                                                                              >  1:578620/3‑100 (MQ=255)                                            acttAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGt                                         >  1:862686/3‑100 (MQ=255)                                                              cctAATCCAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGGGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAAt                       >  1:3808604/3‑100 (MQ=255)                                                                 aaTCAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCaa                    >  1:742566/1‑100 (MQ=255)                                                                    cAAAGAAGAAACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAAttt                 >  1:2764162/1‑100 (MQ=255)                                                                         cagaaACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACa            >  1:6320876/2‑100 (MQ=255)                                                                           gaaACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAAt          >  1:4427512/1‑100 (MQ=255)                                                                           gaaACAGATTGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAAt          >  1:6386383/1‑100 (MQ=255)                                                                             caCAGATTGGGTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAATTa        >  1:2803193/2‑100 (MQ=255)                                                                                  cttGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAATACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAATTAGAAgg   >  1:3787738/2‑100 (MQ=255)                                                                                   ttGGTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAATACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAATTAGAAGGa  >  1:2796657/1‑100 (MQ=255)                                                                                      |                                                                                                 CTAATGCAACAGTTTTTGAAGAAATGTCAAAACCATTTGAACATATTAAACGAATGGAAAGCTTAATCAAAGAAGAAACAGATTGTTTATCAAAACATGCAAATGATTACGATAGTGATACATATAAAACACATATGTCCCGTTATGAATCTTTATCGAATCAATTTGAACAATTAGAAGGA  >  NC_007793/2158037‑2158218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑